A method for purifying epa from fish oil

A technology of EPA-C18 and fish oil, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of slow hydrolysis speed, inability to separate EPA and DHA, and inability to effectively separate EPA and DHA, and achieve recovery High efficiency, not easy to oxidize, easy to operate

Active Publication Date: 2021-04-13
SEPAX TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] This method can selectively hydrolyze triglycerides containing a variety of fatty acids. The rate of hydrolysis of triglycerides containing EPA and DHA by lipase is slower than that of triglycerides without both, thus enriching more unsaturated fatty acid triglycerides, but cannot separate EPA and DHA
[0032] EPA and DHA cannot be effectively separated by a single method, and multiple methods need to be used in combination

Method used

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  • A method for purifying epa from fish oil
  • A method for purifying epa from fish oil
  • A method for purifying epa from fish oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1 Analysis of fish oil crude product before purification

[0100] The crude fish oil is 70 % pure.

[0101] Chromatographic column: Sepax GP-C18 ( 5um, 4.6 x 250 mm )

[0102] mobile phase:

[0103] A: water

[0104] B: Acetonitrile

[0105] Flow rate: 1ml / min Injection volume: 5ul Column temperature: RT

[0106] Sample: 1mg / ml (dissolved in 82% methanol) Pressure: 78bar

[0107] Instrument: Agilent 1260

[0108] Detection wavelength: UV @ 214nm

[0109] Elution was performed according to the following gradient:

[0110] Table 2

[0111] Time min) A% B% 0 5 95 20 0 100

[0112] The crude product analysis diagram is as follows figure 2 shown.

Embodiment 2

[0113] Example 2 Purification of Crude Fish Oil

[0114] The crude fish oil has a purity of 20%.

[0115] Chromatographic column: EPA-C18 ( 1000 x 250 mm )

[0116] mobile phase:

[0117] A: water

[0118] B: Methanol

[0119] Flow rate: 400ml / min Injection volume: 400ml Column temperature: RT

[0120] Sample: 100mg / ml Pressure: 1.2M Pa

[0121] Instrument: Sepax preparative chromatograph

[0122] Detection wavelength: UV @ 214nm

[0123] Gradient: 82%B

[0124] The specific method is as follows:

[0125] (1) Sample weighing: weigh 40g of crude fish oil on an analytical balance;

[0126] (2) Solvent preparation: prepare 400ml of 82% methanol, take 328ml of methanol, and 72ml of purified water, mix the two together, stir evenly, prepare two parts, and set aside;

[0127] (3) Sample dissolution: add one portion of the solvent prepared in (2) to (1) 40g of crude fish oil, seal it with plastic wrap, add a stirrer, and keep stirring on a magnetic stirrer to fully dissolve...

Embodiment 3

[0135] Take 40g of crude fish oil, in which the EPA content is 70% (m / m), add 400ml of 82% methanol (V / V), stir to fully dissolve; put on the chromatographic column EPA-C18 (100mm×250mm , with a packing mass of about 1.18kg), the sample loading flow rate is 200ml / min, after loading the sample is eluted with 82% methanol, the elution flow rate is 400ml / min, and the total elution is 100min, the EPA component solution is collected by HPLC detection, the membrane is concentrated, and the used Nanofiltration membrane area 2.54m 2 , molecular weight cut off 150~300, membrane flux 20L / (m 2 h), the temperature was controlled at 0-50°C, and the concentrated component solutions were dried to obtain 26.2 g of EPA product, with a purity of 97.3% and a recovery rate of 91.1% by HPLC.

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Abstract

The invention relates to the field of analysis, in particular to a method for purifying EPA from fish oil. The method provided by the invention has high selectivity and separation efficiency, and has a good separation effect on EPA, DHA and other impurities; it has certain selective adsorption on unsaturated fatty acids and impurities in fish oil, and has a high sample loading capacity; using organic The mobile phase of solvent or organic solvent / water system, the solvent can be recycled and reused, reducing the cost of industrialization.

Description

technical field [0001] The invention relates to the field of analysis, in particular to a method for purifying EPA from fish oil. Background technique [0002] Eicosapentaenoic acid is a very effective anti-vascular disease drug discovered in recent years, which mainly exists in marine organisms. Eicosapentaenoic acid (EicosaPentaenoic-acid), referred to as EPA, is an omega-3 polyene fatty acid, which can be converted into prostaglandin I: (PGI.) with anticoagulant function in humans and animals and can inhibit thrombus The generation of factor A: (TXA:) has the effect of anti-vascular disease, and its side effects are small, so it is a promising anti-vascular disease drug. EPA is called "vascular scavenger". It has the functions of improving blood circulation, softening blood vessels, regulating blood lipids, lowering blood pressure and blood sugar, and is suitable for middle-aged and elderly people. Clinical trials have affirmed the medicinal effect of EPA, and increasin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C51/47C07C57/03
CPCC07C51/42C07C51/47C07C57/03
Inventor 王艺彭凯黄学英
Owner SEPAX TECH
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