A device for identifying and sorting cumulus cells

Abstract

The invention provides a cumulus cell identification and sorting device, comprising a liquid circuit system, an imaging system and a control system; the liquid circuit system includes an egg retrieval needle, a main input tube, a cell sorting device, a cell buffering device, and a waste liquid buffering device. device, main output line, peristaltic pump, waste tank. The inlet of the cell sorting device is connected with the oocyte retrieval needle through the main input pipe, the first outlet of the cell sorting device is connected with the cell buffer device through the cumulus cell inlet pipe, and the second outlet is connected with the waste liquid through the waste liquid inlet pipe. The buffer device is connected, the main output pipe is connected with the cell buffer device through the first waste liquid outlet pipe, and is connected with the waste liquid buffer device through the second waste liquid outlet pipe, the first waste liquid outlet pipe, the second waste liquid outlet pipe and A three-way valve is arranged between adjacent ports of the main output pipe, and the main output pipe is connected to the peristaltic pump and the waste liquid tank in turn. By using the device provided by the invention, cumulus cells can be automatically identified and sorted, and the accuracy of identification and sorting of cumulus cells can be improved.

Description

technical field [0001] The invention belongs to the technical field of assisted reproduction, and in particular relates to a device for identifying and sorting cumulus cells. Background technique [0002] The collection of cumulus cells plays an important role in the field of assisted reproductive technology and plays an important role in the process of in vitro fertilization. Therefore, how to improve the recovery rate of cumulus cells has become the development direction of assisted reproductive technology. [0003] At present, the collection of cumulus cells mainly adopts the puncture and oocyte retrieval technology guided by transvaginal ultrasound imaging. The detailed process is as follows: (1) Egg retrieval process: the egg retrieval needle is used to puncture the follicle through the vagina under the guidance of the ultrasound probe, and the follicle fluid containing cumulus cells is sucked into the test tube by negative pressure suction. If the test tube is full, ...

AI Technical Summary

Problems solved by technology

[0004] Manual collection of cumulus cells has many disadvantages: First, due to the complex composition of follicular fluid and the very sensitive and fragile cumulus cells, there is a high probability of missed detection, which requires experienced operators

[0005] From the above disadvantages, we can see that the manual extraction of cumulus cells not only has strict requirements on the operator, but also causes great damage to the cumulus cells.

The missed detection rate of manual extraction of cumulus cells is also high

At present, there is still a lack of excellent methods to solve the above problems

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