Plk3 prostatic cancer prognosis detection reagent and kit

A detection kit and a technology for prostate cancer, applied in the biological field, can solve the problems of unpredictable patient metastasis, unknown expression and biological role of Plk3 in prostate cancer, insufficient specificity, etc.

Inactive Publication Date: 2019-02-22
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity of PSA is strong, there are still shortcomings, it cannot accurately predict clinical recurrence, and it cannot predict whether patients will develop metastasis
Moreover, its specificity is insufficient. Studies have shown that various diseases such as benign prostatic hyperplasia, prostatitis, and acute urinary retention can cause an increase in PSA in plasma.
There is no research on the expression and biological role of Plk3 in prostate cancer

Method used

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  • Plk3 prostatic cancer prognosis detection reagent and kit
  • Plk3 prostatic cancer prognosis detection reagent and kit
  • Plk3 prostatic cancer prognosis detection reagent and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] The method of using this kit is as follows:

[0031] (1) Place the slices that have been dewaxed, hydrated, and antigen-restored in the incubation box, and circle the tissue with a water-blocking pen to prevent the reagents from dripping from spreading, and absorb the water on the slices with absorbent paper. , do not touch the tissue, add H 2 o 2 Cover the tissue with 50ul and incubate at room temperature for 30min to block the activity of endogenous peroxidase;

[0032] (2) Rinse with PBS for 3 minutes, repeat 3 times;

[0033] (3) Slightly absorb the PBS solution on the slice with absorbent paper, add 50ul non-immune sheep serum working solution dropwise, and incubate at room temperature for 15 minutes;

[0034] (4) Gently shake off the serum without rinsing, add dropwise 50ul of primary antibody (2μg / ml rabbit-derived anti-Plk3 polyclonal antibody) (Plk3 antibody:PBS=1:100), incubate at room temperature for 60 minutes or overnight at 4°C;

[0035] (5) Rinse wit...

experiment example

[0042] Experimental example: Selection experiment of markers indicative of proliferation and metastasis of prostate cancer

[0043] 1: The expression of Plk3 in prostate cancer and adjacent tissues

[0044] The expression of Plk3 in 68 cases of prostate cancer and 10 cases of normal prostate tissue was detected by immunohistochemical chip. Such as figure 1As shown in A-F, Plk3 is strongly positive in prostate cancer cell plasma and moderately positive in normal prostate tissue. Among the 68 prostate cancer tissues, 19 cases (27.9%) had low expression of Plk3, and 49 cases (72.1%) had high expression of Plk3. And the expression level of Plk3 in prostate cancer tissue was significantly higher than that in normal prostate tissue (IRS: PCa=4.98±0.95vs.Normal=4.30±0.82, P=0.034; T1-T2=4.55±0.90vs.T3-T4= 5.61±0.63, Pfigure 1 B).

[0045] Samples are now fixed in 10% neutral formalin and then embedded in paraffin. Paraffin sections were 4 μm thick, dewaxed in xylene, hydrated wi...

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Abstract

The invention discloses a Plk3 prostatic cancer prognosis detection reagent and kit. The reagent comprises (for 20 people) 1-1.5ml of H2O2, 1-1.5ml of non-immune sheep serum working solution, 2.5-20mul of rabbit Plk3 polyclonal antibodies serving as primary antibodies, 1ml of biotin-labeled goat anti-rabbit IgG working solution serving as secondary antibodies, 1ml of streptomycete antibiotin-peroxidase solution and 2-3ml of DAB color developing agents. The Plk3 prostatic cancer prognosis detection kit comprises the Plk3 detection reagent. Prostatic cancer recurrence can be forecasted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a detection reagent for the prognosis diagnosis of Plk3 prostate cancer, and also relates to the Plk3 detection kit for the prognosis diagnosis of prostate cancer. Background technique [0002] Prostate cancer is the most common malignant tumor of the male reproductive system in the world. For elderly men, its incidence rate ranks second among all malignant tumors. The incidence of prostate cancer varies greatly from country to country. The incidence of prostate cancer in the United States has surpassed that of lung cancer, becoming the highest incidence of male malignancy. Although the incidence of prostate cancer in my country is much lower than that in Europe and the United States, it has shown an obvious upward trend in recent years. [0003] Currently, the main treatment for localized prostate cancer is surgery. However, some patients still have local recurrence an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/64
CPCG01N21/6428G01N33/57434G01N2021/6439
Inventor 黄海郭正辉林春浩赖义明彭圣萌
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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