Germination method for seeds containing endophytic fungi

An endophytic fungus and seed technology, applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of affecting plant growth and low plant bacteria content, and achieve the effect of increasing the bacteria content and seed germination rate

Inactive Publication Date: 2019-02-22
南京胜跃新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when the seeds containing endophytic fungi germinate, the plant's bacterial content is often not high, which affects the growth of plants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Contain the germination of endophytic fungus Fescue chrysanthemum seed:

[0017] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:

[0018] (1) Put 10 grains of fescue seeds containing endophytic fungus into a kraft paper bag, and put the kraft paper bag into a dark environment at 3°C ​​for 24 hours;

[0019] (2) Take out the seeds and place them at room temperature for 1 day;

[0020] (3) Pour the seeds into glass tubes, place 5 seeds per tube, and place them in a water bath environment at 45°C for 10 hours;

[0021] (4) Put the seeds into the hole tray according to 1 seed per hole, and carry out the germination of the seeds of the small glume fescue.

[0022] After testing, the germination rate of the fescue seeds containing the endophytic fungus treated by the method is 90%, and the germination rate of the seedlings is 100%.

Embodiment 2

[0023] Embodiment 2: Contain the germination of endophytic fungus Goose view grass seed:

[0024] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:

[0025] (1) Put 30 grains of E. argentina seeds containing endophytic fungi into a kraft paper bag, and put the kraft paper bag into a dark environment at 6°C for 48 hours;

[0026] (2) Take out the seeds and place them at room temperature for 3 days;

[0027] (3) Pour the seeds into glass tubes, place 15 seeds per tube, and place them in a water bath environment at 55°C for 20 hours;

[0028] (4) Put the seeds into the plug tray according to 2 seeds per hole, and carry out the germination of the seeds of Goose View Grass.

[0029] After testing, the germination rate of the seeds of the endophytic fungus-containing swan grass treated by the method is 91%, and the fungus-containing rate of the seedlings is 99.5%.

Embodiment 3

[0030] Embodiment 3: Contain the germination of endophyte ryegrass seed:

[0031] A kind of seed germination method containing endophytic fungus of the present embodiment is characterized in that: comprises the following steps:

[0032] (1) Put ryegrass seeds containing endophytic fungi into kraft paper bags according to 20 grains per bag, and put the kraft paper bags into a dark environment at 5°C for 36 hours;

[0033] (2) Take out the seeds and place them at room temperature for 2 days;

[0034] (3) Pour the seeds into glass tubes, place 10 seeds per tube, and place them in a water bath environment at 50°C for 15 hours;

[0035] (4) The seeds are put into the hole tray according to 1 seed per hole, and the ryegrass seeds are germinated.

[0036] After testing, the germination rate of the ryegrass seeds containing the endophytic fungus treated by the method is 92%, and the fungus-containing rate of the seedlings is 99.8%.

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PUM

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Abstract

The invention discloses a germination method for seeds containing endophytic fungi. The method is characterized by comprising the following steps: (1) filling the seeds containing endophytic fungi into kraft paper bags according to an amount of 10-30 seeds per bag, and treating the kraft paper bags in a dark environment at 3-6 DEG C for 24-48 hours; (2) taking out the seeds, and placing in a roomtemperature environment for 1-3 days; (3) pouring the seeds into glass guide tubes according to an amount of 5-15 seeds per tube, and treating in a water bath environment of 45-55 DEG C for 10-20 hours; and (4) putting the seeds in a hole tray according to an amount of 1-2 seeds per hole, and germinating the seeds. According to the method disclosed by the invention, the seed germination rate can be increased, and the fungus content of seedlings can also be increased.

Description

technical field [0001] The invention relates to the field of seed germination, in particular to a method for germination of seeds containing endophytic fungi. Background technique [0002] Endophytes are fungi that live in the tissues and organs of healthy plants at certain or all stages, and can provide many benefits to plants, such as cold and drought resistance, promotion of seedling growth and tillering. [0003] However, when the seeds containing endophytic fungi germinate, the fungus rate of the plant is often not high, which affects the growth of the plant. Contents of the invention [0004] Purpose of the invention: Aiming at the deficiencies and defects of the prior art, the present invention provides a method for germination of seeds containing endophytic fungi that can not only increase the germination rate of seeds, but also increase the bacteria content rate of seedlings. [0005] Technical scheme: a method for germination of seeds containing endophytic fungi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 孙嘉斌孙晓哲魏秀慧
Owner 南京胜跃新材料科技有限公司
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