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Method for increasing germination rate of grape seeds

A grape seed and germination rate technology, applied in seed and rhizome treatment, botany equipment and methods, germination equipment, etc., can solve the problems of low germination rate, low seedling rate, and long time required to shorten germination time, increase the reproduction efficiency, and promote the effect of absorption

Inactive Publication Date: 2019-02-22
句容市丁庄葡萄研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding of new grape varieties is mainly based on hybrid breeding and seed selection. However, grape seeds are thick, leathery and have dormancy characteristics. The seed shell contains high abscisic acid or similar, which will inhibit seed germination. , need to undergo low-temperature stratification treatment, and then sow for germination, which takes a long time and the germination rate is not high, and the final seedling rate will be lower, which not only increases the workload of seedling raising, but also delays the process of seedling raising, seriously affecting the Innovation and Utilization of Grape Germplasm Resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for improving grape seed germination rate, comprising the following steps:

[0019] In step 1, dark brown, plump, and shiny mature seeds are selected for freezing pretreatment, and the freezing pretreatment conditions are -2° C. for 2 hours.

[0020] Step 2, place the seeds in step 1 at 20-25°C until the seeds return to normal temperature.

[0021] Step 3: Soak the seeds in step 2 in the complex enzyme solution under the soaking conditions of 30°C for 1 hour, take them out and then soak them in the germination agent under the soaking conditions of 30°C for 1 hour, take them out and dry them to obtain the germination seeds.

[0022] The compound enzyme solution is a mixed aqueous solution of ligninase 1wt.%, cellulase 2wt.%, lipase 0.5wt.%, citric acid 2wt.%. The concentration of each component in the sprouting agent is a mixed aqueous solution of matrine 3 wt.%, 6-BA 2 wt.%, salicylic acid 1 wt.%, and potassium carbonate 2 wt.%.

[0023] Step 4, mix the germi...

Embodiment 2

[0027] A method for improving grape seed germination rate, comprising the following steps:

[0028] In step 1, dark brown, plump and shiny mature seeds are selected for freezing pretreatment, and the freezing pretreatment conditions are 0° C. for 3 hours.

[0029] Step 2, place the seeds in step 1 at 20-25°C until the seeds return to normal temperature.

[0030] Step 3: Soak the seeds in step 2 in the complex enzyme solution under the soaking condition of 35°C for 1 hour, take them out and then soak them in the germination agent at 35°C for 1 hour, take them out and dry them to obtain the germination seeds.

[0031] The compound enzyme solution is a mixed aqueous solution of ligninase 2wt.%, cellulase 2wt.%, lipase 1wt.%, citric acid 3wt.%. The concentration of each component in the sprouting agent is a mixed aqueous solution of matrine 4wt.%, 6-BA 2 wt.%, salicylic acid 2wt.%, potassium carbonate 3 wt.%.

[0032] Step 4, mix the germinated seeds with wet river sand at a wei...

Embodiment 3

[0036] A method for improving grape seed germination rate, comprising the following steps:

[0037] In step 1, dark brown, plump, and shiny mature seeds are selected for freezing pretreatment, and the freezing pretreatment conditions are -2° C. for 2 hours.

[0038] Step 2, place the seeds in step 1 at 20-25°C until the seeds return to normal temperature.

[0039] Step 3: Soak the seeds in step 2 in the complex enzyme solution under the soaking conditions of 30°C for 1 hour, take them out and then soak them in the germination agent under the soaking conditions of 30°C for 1 hour, take them out and dry them to obtain the germination seeds.

[0040] The compound enzyme solution is a mixed aqueous solution of ligninase 1.5wt.%, cellulase 1.5wt.%, lipase 0.5wt.%, and citric acid 4wt.%. The concentration of each component in the sprouting agent is a mixed aqueous solution of matrine 4 wt.%, 6-BA 1.5 wt.%, salicylic acid 1.5 wt.%, and potassium carbonate 4 wt.%.

[0041] Step 4, m...

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PUM

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Abstract

The invention discloses a method for increasing the germination rate of grape seeds. The method comprises the following steps: performing freeze pretreatment on plump and glossy ripe seeds with dark brown color, and standing the seeds at 20-25 DEG C until the seeds are restored to normal temperature; sequentially soaking the seeds in a complex enzyme solution and a germinating agent, taking out, and drying in the air; and mixing the germinated seeds with wet river sands, preserving in an environment having the temperature of 10-15 DEG C and relative humidity of 85-90 percent in a closed manner, wherein the complex enzyme solution is a mixed solution of ligninase, cellulose, lipase and citric acid; and the germinating agent is a mixed solution of matrine, 6-BA, salicylic acid and potassiumcarbonate. The method can destroy the seed coat structure, quickly soften the seed coats and promote seeds to absorb external nutrition, so that the germinating time of seeds can be shortened, and thebreeding efficiency of seeds can be improved.

Description

technical field [0001] The invention belongs to the technical field of grape planting, and in particular relates to a method for improving the germination rate of grape seeds. Background technique [0002] Grapes are widely cultivated. The world's cultivated area and output of grapes are second only to citrus. They have important economic value and are favored by consumers because of their good appearance, flavor and rich nutrition. At present, the breeding of new grape varieties is mainly based on hybrid breeding and seed selection. However, grape seeds are thick, leathery and have dormancy characteristics. The seed shell contains high abscisic acid or similar, which will inhibit seed germination. , need to undergo low-temperature stratification treatment, and then sow for germination, which takes a long time and the germination rate is not high, and the final seedling rate will be lower, which not only increases the workload of seedling raising, but also delays the proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/02A01N63/00A01N59/00A01N43/90A01N37/40A01P21/00
CPCA01C1/00A01C1/02A01N37/40A01N43/90A01N59/00A01N63/00
Inventor 汲美花汲静毛伟孝汲元浩慷芮冬明董香楠徐曼李伟铭汲科言李伦牟悦锐门洪宇刘光
Owner 句容市丁庄葡萄研究所
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