an afft1 cell

Abstract

The application relates to AFFT1 cell and a preparation method thereof. The RFF cell is reformed by using TCR-T technical principle, the knock-out of the immunosuppression target spot is performed onthe reformed T cell by using gene editing technology, the condition that the specific lysis T cell is prevented from in-vivo suppression is precisely protected, and the lethality on the tumor cell bythe T cell is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an AFFT1 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proven to be ineffective, while NK, CAR-NK, TIL, and other cell technologies have yet to mature. CAR - T cells have shortcomings in safety and solid tumor therapy. [0003] In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0004] The above treatment methods are immature, especially the in vitro induction of DC ce...

AI Technical Summary

Problems solved by technology

[0005] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful immune microenvironment

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