Fermentation process research of bacillus laterosporus capable of degrading organophosphorus pesticide

Abstract

The invention provides a fermentation process research of bacillus laterosporus capable of degrading organophosphorus pesticide. The fermentation process research is finished by adopting the followingsteps: firstly, preparing seed liquid; secondly, preparing a culture medium of a isocarbophos-containing pesticide and inoculating; thirdly, determining the degradation rate of the isocarbophos; fourthly, researching the characteristics of experimental strains for degrading the organophosphorus pesticide; fifthly, researching the condition of degrading the isocarbophos by using the experimental strains; sixthly, researching the degradation of organophosphorus pesticide enzymes by using the experimental strains; seventhly, researching degradation products and a degradation pathway of the isocarbophos; eighthly, carrying out use safety experiment of the strain. The research provided by the invention researches the degradation effect of the strain on the organophosphorus pesticide accordingto the concentration of pesticide sprayed in farmland, so that the influence of pesticide spraying dose on strain production through biologic bacterial fertilizer and the degradation ability of the strain to the organophosphorus pesticide are further known.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to the research on the fermentation process of Bacillus lateralis which can degrade organophosphorus pesticides. Background technique [0002] Since the appearance of organophosphorus pesticides, its production and application have a history of more than 70 years. As a class of high-efficiency and broad-spectrum pesticide preparations, organophosphorus pesticides have been widely used in agricultural production, which has greatly increased the yield of crops, and has become an important guarantee for increasing global agricultural production and income. contribute significantly to shortages. At present, there are hundreds of kinds of organophosphorus pesticides in the world, and they are the most widely used pesticides at home and abroad. my country is a big agricultural country, and it is also the country that uses the largest amount of pesticides in t...

AI Technical Summary

Problems solved by technology

[0008] In conclusion, great progress has been made in the research of microbial degradation of organophosphorus pesticides, but there are still many difficulties in practical application due to the particularity of organophosphorus pesticide pollution.