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Method for screening, separating and purifying algae species of Haematococcus pluvialis

A technology for separation and purification of Haematococcus pluvialis, which is applied in the field of screening, separation and purification of algae species of Haematococcus pluvialis, to achieve the effects of enhancing tolerance and resistance, good application prospects, and increasing cell culture density

Inactive Publication Date: 2019-02-01
云南博欣生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the screening, separation and purification of algae strains of Haematococcus pluvialis;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0038] Example 1. Production of special capillary pipettes for the selection of Haematococcus pluvialis cells

[0039] 1. Choose the German Brand glass Pasteur pipette, model 747715, with a total length of 145mm, a tip length of 45mm, and a capacity of about 2ml;

[0040] 2. Burning, use the middle and outer flame of the alcohol lamp to control the temperature to burn the tip of the Pasteur pipette;

[0041] 3. Shake (shake), keep shaking during the burning process to ensure that the tip is evenly heated;

[0042] 4. Take it out, and when the tip part is evenly burned red, take it out from the alcohol lamp;

[0043]5. Pull, use tweezers to pull out, ensure that the length of the pull is about 5cm, and the diameter of the wire drawing is about 60nm;

[0044] 6. Stop to ensure that the wire drawing length and aperture meet the requirements and stop the drawing immediately;

[0045] 7. Break, after stopping, forcefully break off the outer end of the tweezers to ensure that the...

example 2

[0046] Example 2. Screening, separation and purification of individual algal cells from Haematococcus pluvialis

[0047] 1. Prepare a 50ml centrifuge tube in advance, sterilize it at 121°C for 30 minutes, and dry it for later use;

[0048] 2. Select Haematococcus pluvialis that divides vigorously in the outdoor culture environment, is in the division stage, and has strong adaptability. Use a sterilized 50ml centrifuge tube for sampling. The sampling time is generally 8:00-10:00 in the morning During the sampling, the first three rinses were washed and poured out, and the algae liquid mixed evenly for the fourth time was taken;

[0049] 3. The production method and sterilization treatment of capillary straws: while sampling, another person is responsible for making capillary straws, using glass Pasteur straws to clean ultrasonically, soak in acid, soak in alcohol, dry, and dry at 115 Sterilize at ℃ for 2 hours, use an alcohol blowtorch to burn and draw, and draw the Pasteur pi...

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Abstract

A method for screening, separating and purifying single algae cells of Haematococcus pluvialis includes the following steps: preparation of capillary micropipettes for Haematococcus pluvialis cells, single high-quality cell screening, separation and purification measures, expanding of culture, rejuvenation, and culture solution preparation. An algae liquid with good growth vigor of Haematococcus pluvialis, short short cell adaptation period, strong division ability, large cell diameter and strong resistance in the outdoor tube reactor is selected. The method mainly comprises the screening, separation and purification steps of single algae cells. By the method, tolerance and resistance of high-quality Haematococcus pluvialis are enhanced; through repeated washing of high-quality cells, purity of the cells is ensured; the equipment for each separation of the obtained high-quality cells has low capacity; the culture time is short; operation is simple; and the problem that the probabilityof success is low in the contamination and transfer of Haematococcus pluvialis and work efficiency is affected is avoided.

Description

technical field [0001] The invention relates to a method for screening, separating and purifying algae species of Haematococcus pluvialis, which is a new method for efficiently screening the algae species of Haematococcus pluvialis. Background technique [0002] The cultivation and application of microalgae first require algae species. The algae species are first screened and purified from the mixed biota in natural waters, and obtained by pure species of single algae culture, and then expanded and preserved for experimental production. Screening and purifying microalgae is an indispensable technology for its scientific research. [0003] With the development trend of artificial large-scale cultivation of Haematococcus pluvialis, according to the changes in the local climate and environment during the cultivation process, if the algae activity is not good, the adaptation period is very long, the logarithmic period is shortened, the yield is not high, shrimp The algae speci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 李路荣徐蕾仲林强柏老六
Owner 云南博欣生物科技股份有限公司
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