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Sample placing system and method for acquiring single-particle fluorescence-microstructure

A micro-morphology and single-particle technology, which is applied in the research fields of single-particle fluorescence spectroscopy and micro-morphology analysis, can solve problems such as the inability to obtain single-particle dispersed samples, reduce fluorescence background noise, improve resolution, and improve microscopic The effect of topography resolution

Active Publication Date: 2019-01-25
SHANDONG UNIV
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  • Abstract
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Problems solved by technology

[0005] (3) The traditional quartz glass slide has sufficient mechanical strength, but the copper mesh / carbon film used in the traditional transmission electron microscope cannot be used for high-speed spin-coating sample preparation, and thus cannot obtain single-particle dispersed samples

Method used

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  • Sample placing system and method for acquiring single-particle fluorescence-microstructure
  • Sample placing system and method for acquiring single-particle fluorescence-microstructure
  • Sample placing system and method for acquiring single-particle fluorescence-microstructure

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Embodiment Construction

[0046] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0047] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0048] As introduced in the background technology, there are deficiencies in the prior art. In order to solve th...

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Abstract

The invention discloses a sample placing system and method for acquiring single-particle fluorescence-microstructure. The system comprises a first sealing ring, a slide, a second sealing ring, a coverpiece, a fixed ring, a thin film window for transmission electron microscope and a spacer, wherein a single-particle sample is loaded on a lower surface of the thin film window. When used for single-particle fluorescent measurement, the whole set of sample placing system is placed above an objective lens of a concentration fluorescent microscopy, and fluorescent spectrum and a fluorescent image can be acquired; and when used for microstructure analysis, the thin film window in the system is taken out, a transmission electron microscope sample rod is reversely placed, representation is achieved by a transmission electron microscope, and a microstructure map of a corresponding region can be obtained. Single-particle fluorescence and microstructure analysis can be performed on the same nanoparticle, and the system has the characteristics of clean single-particle fluorescent signal and high microstructure resolution.

Description

technical field [0001] The invention relates to the research field of single particle fluorescence spectrum measurement and microscopic shape analysis, in particular to a sample setting system and a use method for obtaining single particle fluorescence-microscopic shape. Background technique [0002] Single particle fluorescence spectroscopy, based on laser scanning confocal microscopy, can reveal the energy relaxation mechanism of a single nanoparticle and monitor the photochemical reaction process on its surface in real time, and has become an effective means to study the charge transfer mechanism and catalytic reaction mechanism . However, traditional single-particle fluorescence spectroscopy cannot provide high-resolution microscopic morphology information at the same time, which limits the research on charge transfer mechanism and photochemical reaction process in fine nanostructures. The difficulty lies in: [0003] (1) The acquisition of high-resolution microscopic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6458
Inventor 郑昭科黄柏标张晓阳秦晓燕王泽岩王朋刘媛媛张倩倩
Owner SHANDONG UNIV
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