Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human papillomavirus DNA typing detection kit

A human papillomavirus and DNA typing technology, which is applied in the field of human papillomavirus DNA typing detection kits, can solve the problem that low-risk HPV viruses cannot be ruled out to cause cancer, etc.

Inactive Publication Date: 2019-01-18
上海裕隆神光医学检验实验室有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low-risk HPV viruses can also cause diseases, and the possibility of low-risk HPV viruses causing cancer cannot be ruled out

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0174] The present embodiment provides the DNA typing detection kit of human papillomavirus, the composition of described kit is as follows:

[0175] 1. DNA extraction solution, 5mL / bottle, 1 bottle, the main components are 25mM NaOH, 10mM Tris-HCl (pH8.0), 1%TritonX-100, 1% NP-40, 2% Chelex-100, 0.1mM EDTA (pH8.0);

[0176] 2. Taq / UNG enzyme mixture, 215μL / tube, 1 tube, the main component is a 100:2 (volume ratio) mixture of 2.5U / μL Taq DNA polymerase and UNG enzyme;

[0177] 3. PCR reaction solution of HPV16, HPV35, HPV58 and HPV61, 1mL / tube, 1 tube, the main components are PCR buffer (50mM KCl, 50mM (NH 4 ) 2 SO 4 , 100mM Tris-HCl (pH8.5)), 8mM dATP, 8mM dGTP, 8mM dCTP, 4mM dTTP, 4mM dUTP, 10mM MgCl 2 , primers and probes of HPV16, primers and probes of HPV35, primers and probes of HPV58 and primers and probes of HPV61, wherein each primer is 80 μM, and each probe is 40 μM;

[0178] 4. PCR reaction solution of HPV18, HPV33, HPV51 and HPV73, 1mL / tube, 1 tube, the main c...

Embodiment 2

[0187] This embodiment provides a method for using the kit in the above-mentioned embodiment 1 to detect the DNA typing of human papillomavirus in a sample, which includes the following steps:

[0188] (1) Collect and store samples

[0189] The tested samples are cervical exfoliated cells of human subjects.

[0190] Sampling method: soak a cotton swab in normal saline, gently wipe the cervical secretions with the cotton swab, put the cervical sampling brush into the cervical opening, turn it slightly for 3 to 5 weeks, and then take out the cervical sampling brush. Put it in a sample tube with cell preservation solution. Break off the brush handle at the mouth of the tube, leave the brush head in the sample tube, tighten the tube cap, and send the sample for inspection immediately.

[0191] Sample storage: The collected samples should be sent for inspection as soon as possible. It is recommended to store them at 4°C for no more than 10 hours, and at -20°C for no more than 11 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a human papillomavirus DNA typing detection kit, including DNA extracts, Taq / UNG enzyme mixture, positive control, negative control, HPV16, HPV35, PCR reaction solution of HPV58 and HPV61, HPV18, HPV33, PCR reaction solution of HPV51 and HPV73, HPV39, HPV45, PCR reaction solution of HPV56 and HPV59, HPV31, HPV52, PCR reaction solutions of HPV53 and HPV81, PCR reaction solutions of HPV6, HPV42, HPV70 and HPV72, PCR reaction solutions of HPV11, HPV43, HPV44 and HPV82, and PCR reaction solutions of HPV26, HPV40, HPV54, HPV83 and human beta; The PCR reaction solution of globulin. The kit of the invention adopts fluorescence. PCR can detect many DNA types of human papillomavirus at the same time, which has important guiding significance for clinic.

Description

technical field [0001] The invention relates to the technical field of viral nucleic acid detection, in particular to a DNA typing detection kit for human papillomavirus. Background technique [0002] Cervical cancer is one of the most common cancers in the human body. It not only occupies the first place among female reproductive organ cancers, but also is the most common cancer among various female malignant tumors. The incidence of cervical cancer in my country tends to be higher than that in rural areas. Cities, mountains are higher than plains. According to retrospective investigations in 29 provinces, municipalities, and autonomous regions, the mortality rate of cervical cancer in my country ranks fourth among all cancers and ranks second among female cancers. [0003] HPV is Human papilloma virus (Human papilloma virus), more than 130 types of HPV have been isolated so far, and can be divided into four types according to the different tissue parts it invades: skin low...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/708C12Q2545/113C12Q2521/531
Inventor 穆海东汪宁梅
Owner 上海裕隆神光医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com