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Detection method of pumpkin place of origin

A detection method and technology for pumpkins, which are applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of classification, difficult pumpkin origin, etc., and achieve the effect of convenient operation and intuitive map.

Inactive Publication Date: 2019-01-15
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem in the prior art that it is difficult to accurately classify pumpkin origins through plant morphological characteristics, the present invention provides a detection method for pumpkin origins

Method used

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  • Detection method of pumpkin place of origin
  • Detection method of pumpkin place of origin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for constructing the enzyme electrophoresis reference fingerprint of pumpkin comprises:

[0033] (A) Take 1 g of pumpkin root, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0034] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0035] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0036] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow electrophoresis, the current of ...

Embodiment 2

[0038] The method for constructing the enzyme electrophoresis reference fingerprint of pumpkin comprises:

[0039] (A) Take 1 g of pumpkin stem, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize it in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0040] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0041] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0042] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow electrophoresis, the current ...

Embodiment 3

[0044] The method for constructing the enzyme electrophoresis reference fingerprint of pumpkin comprises:

[0045] (A) Take 1 g of pumpkin leaves, add 3 mL of phosphate buffer solution with a concentration of 0.05 mol / L, homogenize in a pre-cooled mortar and centrifuge, take the supernatant, add glycerin and shake well to obtain the enzyme electrophoresis solution and store it at low temperature ;

[0046] (B) using a gel electrophoresis system to perform electrophoresis on the enzyme electrophoresis solution described in step (A);

[0047] (C) Mapping the pattern and counting the number of bands based on the results of step (B).

[0048] The pH of the phosphate buffer described in the step (A) is 6.8, the added amount of glycerol is 20 (wt)%, and the temperature for cryopreservation is -20°C; the gel electrophoresis system described in the step (B) is a discontinuous acrylamide gel Gel electrophoresis system; the electrophoresis is steady flow electrophoresis, the current o...

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Abstract

The invention provides a detection method of pumpkin place of origin. The method comprises the following steps: firstly constructing enzyme electrophoresis reference fingerprints of pumpkins of different known places of origin, and establishing the enzyme electrophoresis reference fingerprint of the to-be-detected pumpkin place of origin by using the same method, and then performing clustering analysis on the enzyme electrophoresis reference fingerprints and the enzyme electrophoresis reference fingerprint, computing a fingerprint dissimilarity coefficient C to obtain the place of origin of the to-be-detected pumpkin, wherein a migration rate Rf of the fingerprint is more than 0.2, the dissimilarity coefficient C is equal to 2w / (a+b), w represents enzyme band number with different two-phase comparison type in the to-be-detected pumpkin place of origin and the known pumpkin place of origin, and (a+b) represents the total enzyme band of respective two-phase comparison type. Through the detection method of pumpkin place of origin provided by the invention, the operation is convenient, and the atlas is visual, the pumpkin place of origin can be objectively and accurately reflected andclassified. The method is free from the limitation of pumpkin shape, size and growth time, the different parts such as the root, the stems, the leaves, the fruits, and the seeds of the pumpkin can bedetected so as to obtain the pumpkin place of origin.

Description

technical field [0001] The invention belongs to the technical field of pumpkin planting, and in particular relates to a method for detecting the origin of pumpkins. Background technique [0002] Pumpkin (scientific name: Cucurbita moschata (Duch.ex Lam.) Duch.ex Poiret) is a species of Cucurbita moschata (Duch.ex Lam.) Duch.ex Poiret, an annual vine herb, with stems often rooting at nodes, thick petioles, broad oval or oval leaves, Slightly soft, with raised veins, slightly thick tendrils, monoecious, strong fruit stems, with ribs and grooves, which vary from variety to variety, and there are often several longitudinal grooves outside, many seeds, long oval or oblong, with good food therapy effect. [0003] Pumpkin cultivars are rich in resources. In the prior art, most of them are classified by plant morphological characteristics. Plant morphology is to study the internal and external morphology and structure of plants, the formation and development of organs, cells, tissu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 何丽烂任吉君王惠珍区炳庆
Owner FOSHAN UNIVERSITY
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