Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A Molecular Marker Linked to Capsicum Green Retention Gene and Its Application

A technology of molecular marker and green retention gene, applied in the field of pepper breeding and molecular biology, can solve the problems of unsatisfactory green preservation effect and high cost, and achieve the goal of reducing planting scale, reducing workload, and improving efficiency and accuracy. Effect

Active Publication Date: 2021-07-23
HUNAN VEGETABLE RES INST
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, although there are many ways to keep vegetables green, some methods cost too much, and some methods are restricted by the Food Sanitation Law due to the residue of heavy metals in the processing process, and the green keeping effect is not very ideal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Molecular Marker Linked to Capsicum Green Retention Gene and Its Application
  • A Molecular Marker Linked to Capsicum Green Retention Gene and Its Application
  • A Molecular Marker Linked to Capsicum Green Retention Gene and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Acquisition of the Molecular Marker SGR Molecular Marker Linked to the Capsicum Chlorophyllin Gene

[0045] BSR positioning method

[0046] 1. Group construction

[0047] The material 'CT2' (such as figure 2 shown on the left), and the material 'CS17' whose fruit color is brown is the parent (such as figure 2 Shown on the right side of the middle), all are resequenced pepper germplasm resources, the brown parent and the red parent were crossed to obtain the F1 generation, and the F1 generation was self-crossed to obtain the F2 population.

[0048] 2. Fruit color identification

[0049] After the fruit is ripe, the brown / red color of the fruit is identified.

[0050] 3. Fruit color main effect QTL analysis

[0051] F in CT2 x CS17 2 In the group, 30 plants with red fruit color and 30 mature fruits with brown fruit color were selected, and the fruits of each plant were mixed in equal amounts to construct a red fruit / brown fruit RNA mixed pool. The total...

Embodiment 2

[0056] Example 2 Verification of mature capsicum fruit color marking SGR

[0057] Using the molecular marker primers obtained for the color of the fruit, F 2 90 individual plants of the population carry out fruit color identification, and its steps are as follows:

[0058] (1) Carry out touchdown PCR reaction with pepper genomic DNA as a template; add 3 primers labeled with SGR to the reaction system, the sequence of the universal reverse primer labeled with SGR is SGR-1 (SEQ ID NO.1), and the two primers labeled with SGR A forward primer sequence is SGR-2 (SEQ ID NO.5) connected with a fluorescent linker sequence, and SGR-3 (SEQ ID NO.6) connected with a fluorescent linker sequence. Touchdown PCR reaction system: the total volume is 3 μl, and the specific components are as follows: ddH 2 O 1.4983μl, 2×KASPMasterMix 1.4792μl, two forward primers SGR-2 (SEQ ID NO.5) and SGR-3 (SEQ ID NO.6) connected with the fluorescent adapter sequence each 0.005μl (100uM), reverse To prime...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker linked with a capsicum stagnation gene and its application. F was constructed using 2 highly homozygous materials 2 group. Chromosomal regions closely linked to pepper fruit brown (green gene) were obtained by BSR population mapping method, and molecular markers were developed in the candidate interval to determine candidate genes. A KASP molecular marker was designed according to the single base mutation of the candidate gene, and the marker was used to pair F 2 90 individual plants in the population were genotyped, and the coincidence rate reached 100%. The research results are not only helpful for pepper fruit color identification and assisted breeding, but also provide a basis for the map-based cloning of stagnant green genes and analysis of the molecular mechanism of stagnant green, which has extensive promotion value.

Description

technical field [0001] The invention belongs to the fields of capsicum breeding and molecular biology, and relates to a molecular marker linked with a capsicum (Capsicum annuum L) green-sustaining gene and the application of the molecular marker in capsicum fruit color identification and breeding. Background technique [0002] Capsicum, Latin name: Capsicum annuum L., Solanaceae, Capsicum genus annual or limited perennial herb. Capsicum mainly includes 5 cultivars: C.annuum L, C.frutescens L, C.baccacum L, C.pubescensKeep, C.chinese Jacquin. Chili peppers are native to the tropical regions of Central Latin America, and the country of origin is Mexico. In China, it is mainly distributed in Sichuan, Guizhou, Hunan, Yunnan, Shaanxi, Henan (Xichuan County), Jize County of Hebei Province and Tuoketuo County of Inner Mongolia. According to the statistics of the bulk vegetable system of the Ministry of Agriculture, the cultivation area of ​​pepper in my country is increasing cont...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/158
Inventor 欧立军刘宇华杨博智陈文超张竹青周书栋杨莎李雪峰马艳青戴雄泽邹学校
Owner HUNAN VEGETABLE RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com