Application of zmrph1 gene in regulation of plant height and lodging resistance

A lodging resistance and genetic technology, applied in the fields of biotechnology and plant genetics and breeding, to achieve the effects of improving lodging resistance, improving corn harvest index, and high-yield and stable-yield corn harvest index

Active Publication Date: 2021-01-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are relatively few studies on cytoskeleton-related proteins that regulate microtubules and microfilaments in maize, and their application to crop improvement in reducing maize plant height has not been reported

Method used

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  • Application of zmrph1 gene in regulation of plant height and lodging resistance
  • Application of zmrph1 gene in regulation of plant height and lodging resistance
  • Application of zmrph1 gene in regulation of plant height and lodging resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Construction and genetic transformation of ZmRPH1 expression vector

[0053] Total RNA was extracted from corn inbred line B73 V3 stage seedlings, and cDNA was synthesized by reverse transcription using cDNASynthesis Kit from Takala Company, and amplified with specific primers to obtain the CDS sequence of ZmRPH1, with a full length of 1650bp (SEQ ID NO: 1). The nucleotide sequence of the primer pair is:

[0054] Upstream primer: ZmRPH1-F:5'-ATGGTGGCTGCCGCGGCG-3'

[0055] Downstream primer: ZmRPH1-R:5'-CTACAATTGTGTCGGGCTCTGCTTT-3'

[0056] The PCR product was recovered, the target fragment was connected to the pBCXUN vector, and Escherichia coli competent cells were transformed to obtain the recombinant vector pBCXUN-ZmRPH1 with correct sequencing.

[0057] The expression cassette is composed of the ZmUbiquitin1 promoter and the 3' transcription termination region from the nopaline synthase (nos) gene, and the selectable marker gene is phosphinothricin. Its...

Embodiment 2

[0060] Embodiment 2 Preparation of hybrids

[0061] Using the material of the inbred line B73-329 background (the transgenic line that overexpresses the ZmRPH1 gene of the homozygous B73-329 background) as the female parent, and the maize inbred line T13 as the male parent, cross breeding to obtain a hybrid that overexpresses ZmRPH1 kind of material.

Embodiment 3

[0062] Example 3 Analysis of tissue expression pattern of ZmRPH1

[0063] The total RNA of multiple tissues and organs (root, node, internode, leaf, ear, filament, tassel) in four different growth stages (V9, V11, V13, V15) of maize inbred line B73-329 were extracted, Reverse transcribed into cDNA. Using ZmRPH1 gene-specific primers (upstream primer: ZmRPH1qPCR-F:5'-CAAAGTTGCAACGCAAGAAC-3'

[0064] Downstream primer: ZmRPH1qPCR-R:5'-GGCTCTGCTTTTGGTTACGTT-3') for real-time fluorescent quantitative PCR amplification, with ZmActin1 gene as an internal reference gene, and its specific primers are: upstream primer: Zmactin1qPCR-F:5'-TTGTCCTCAGTGGGGGATCT-3' Downstream primer: ZmActin1qPCR-R: 5'-CTTTCAGGTGGCGCAATCAC-3'. The result is as figure 1 As shown, it was shown that ZmRPH1 was highly expressed in the internode tissue in the stage of rapid elongation.

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Abstract

The invention discloses application of a ZmRPH1 gene to plant height and lodging-resistant capability regulation and control, wherein the sequences of the ZmRPH1 gene and ZmRPH1 gene coded protein arerespectively shown as SEQ ID NO: 1 and 2. The condition that the ZmRPH1 gene has the biological function of plant height and lodging-resistant capability regulation and control is discovered for thefirst time. The corn plant height can be regulated and controlled through the ZmRPH1 expression regulation and control in corn; through ZmRPH1 gene expression, the corn plant height can be reduced; the corn lodging-resistant capability is improved without influencing the single plant yield. The invention provides a novel method for reducing the corn plant height; the method can be used for culturing the semi-dwarf corn new variety. The high yield and stable yield of the corn are facilitated; the corn harvesting index can be improved; the corn lodging-resistant capability is enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology and plant genetic breeding, in particular to the application of ZmRPH1 gene in regulating plant height and lodging resistance. Background technique [0002] As one of the most important components of ideal plant type, plant height plays a vital role in crop improvement (Donald, 1968). Appropriate plant height is conducive to improving the harvest index, enhancing lodging resistance, and achieving high and stable yields. These advantages have been fully utilized in the breeding process of wheat and rice. The so-called "green revolution" is the huge yield improvement brought about by the dwarf breeding of rice and wheat, which specifically involves the utilization of sd1 in rice and rht1 in wheat (Peng et al., 1999). However, so far, there is still no available gene that can effectively reduce plant height in maize without causing adverse effects on yield traits. Therefore, it is of great theoretical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 傅缨李围朱蕾王喜庆
Owner CHINA AGRI UNIV
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