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Palm leaf extract and application thereof

A kind of technology of leaf extract and palm tree, which is applied in the application field of preparing antidepressant drugs

Active Publication Date: 2019-01-15
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the antidepressant effect of the leaves of the flower palm

Method used

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  • Palm leaf extract and application thereof
  • Palm leaf extract and application thereof
  • Palm leaf extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation of embodiment 1 flower palm wood leaf extract

[0015] Take 1000g of dry palm leaves raw material, crush them, add ethanol to the crushed leaves at 30°C for 3 times at 30°C according to the ratio of 1g of leaves plus 12mL of ethanol with a mass concentration of 70%, After 30 minutes, the extracts were suction-filtered and combined, and the ethanol was recovered under reduced pressure at 55°C to obtain 2L of an aqueous solution; then the aqueous solution was extracted with petroleum ether until the ether layer was colorless, the petroleum ether layer was discarded, the pH of the aqueous layer was adjusted to 3, and the pH of the aqueous layer was adjusted to 3. The flow rate of 4BV / h passes through the chromatographic column equipped with 800ml AB-8 macroporous adsorption resin. Elute at a flow rate of 3-4BV / h, collect the eluate, recover ethanol under reduced pressure at 55°C, and then freeze-dry to obtain the extract of the leaves of the flower palm.

Embodiment 2

[0016] The preparation of embodiment 2 flower palm wood leaf extract

[0017] Take 1000 g of dry palm leaves raw material, crush them, add ethanol to the crushed leaves at 35°C for 3 times at 35°C according to the ratio of 1 g of leaves plus 10 mL of ethanol with a mass concentration of 70%, After 30 minutes, the extracts were suction-filtered and combined, and ethanol was recovered under reduced pressure at 50°C to obtain an aqueous solution; then the aqueous solution was extracted with petroleum ether until the ether layer was colorless, the petroleum ether layer was discarded, the pH of the aqueous layer was adjusted to 2.5, and the pH of the aqueous layer was adjusted to 2.5. The flow rate of / h passes through the chromatographic column equipped with 800ml AB-8 macroporous adsorption resin, after loading the sample, let it stand for 30min, wash it with water until the effluent is colorless, then use 4000ml mass concentration of 70% ethanol with 3 Elute at a flow rate of -4...

Embodiment 3

[0018] The preparation of embodiment 3 flower palm wood leaf extract

[0019] Take 1000 g of dry palm leaves raw material, crush them, and add ethanol to the crushed leaves at 25°C for 3 times at 25° C. After 30 minutes, the extracts were suction-filtered and combined, and the ethanol was recovered under reduced pressure at 52°C to obtain an aqueous solution; then the aqueous solution was extracted with petroleum ether until the ether layer was colorless, the petroleum ether layer was discarded, the pH of the aqueous layer was adjusted to 3.5, and the pH of the aqueous layer was adjusted to 3.5. The flow rate of / h passes through the chromatographic column equipped with 800ml AB-8 macroporous adsorption resin, after loading the sample, let it stand for 30min, wash it with water until the effluent is colorless, then use 4000ml mass concentration of 70% ethanol with 3 Elute at a flow rate of -4BV / h, collect the eluate, recover ethanol under reduced pressure at 52°C, and then fre...

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Abstract

A palm leaf extract is prepared by ultrasonic extracting pulverized palm leaf with ethanol, and establishing mouse depression model by chronic unpredictable stress to study the antidepressant effect of palm leaf extract. The results of sugar-water preference test, open-field test, novelty environment inhibition feeding test and hanging-tail behavior test showed that palm wood extract had obvious antidepressant effect, which provided a basis for clinical research.

Description

technical field [0001] The present invention relates to the leaf extract of japonica and also relates to the application of the extract in the preparation of antidepressants. Background technique [0002] Flower palm belongs to Fabaceae Papilionaceae red bean plant, widely distributed in Anhui, Zhejiang, Jiangxi, Hubei, Hunan, Guangdong, Sichuan, Guizhou and Yunnan and other places. Palm wood is a tree species for high-end furniture, carvings and precious decorations. It is also a kind of Chinese medicinal material. According to the "National Chinese Herbal Medicine Compilation", its roots, stems, bark, leaves and other tree organs can be used as medicine. For bruises, lumbar muscle strain, rheumatic joint pain, postpartum blood stasis pain, leucorrhea. So far, there has been no report on the antidepressant effect of the leaves of the flower palm tree. Contents of the invention [0003] The object of the present invention is to provide a kind of Fructus Alba Leaf Extract...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P25/24A61K127/00
CPCA61K36/48A61K2236/333A61K2236/35A61K2236/39A61K2236/55A61P25/24
Inventor 唐其陆英朱仕豪
Owner HUNAN AGRICULTURAL UNIV
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