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Method for differentiating oolong tea with different producing areas and fermentation types

An oolong tea, type technology, applied in the direction of material separation, analysis materials, measuring devices, etc., to achieve accurate evaluation results

Inactive Publication Date: 2019-01-08
云南清莲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the method of using the information of the aroma components of each tea and further using chemometric methods (CA and PCA) to distinguish oolong teas from different production areas and fermentation types

Method used

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  • Method for differentiating oolong tea with different producing areas and fermentation types
  • Method for differentiating oolong tea with different producing areas and fermentation types
  • Method for differentiating oolong tea with different producing areas and fermentation types

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: A kind of method utilizing full-automatic headspace solid-phase microextraction in conjunction with gas chromatography mass spectrometry and chemometric method to distinguish different production areas of oolong tea, concrete steps are as follows:

[0045] (1) Material preparation: 5 oolong tea samples are from Fujian Province, numbered FO1-FO5, 2 oolong tea samples are from Taiwan, numbered TO1-TO2, and the production year of all teas is 2013.

[0046] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in the incubator of the headspace sampler at a speed of 250r / min. After equilibrating at 80°C for 10min, the autosampler inserts the extraction head into the headspace part of the headspace ...

Embodiment 2

[0049] Embodiment 2: a kind of method utilizing automatic headspace solid-phase microextraction in conjunction with gas chromatography mass spectrometry and chemometrics method to distinguish different fermented types of oolong tea, concrete steps are as follows:

[0050] (1) Material preparation: 5 lightly fermented oolong tea samples were from Fujian Province, numbered QO1-QO5. 3 heavily fermented oolong tea samples were from Fujian, Guangdong and Taiwan, numbered ZO1-ZO3, and the production years were year 2013.

[0051] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in the incubator of the headspace sampler at a speed of 250r / min. After equilibrating at 80°C for 10min, the autosampler inserts the extra...

Embodiment 3

[0054] Embodiment 3: a kind of method utilizing full-automatic headspace solid-phase microextraction in combination with gas chromatography mass spectrometry and chemometrics method to distinguish the Tieguanyin produced in Fujian and other oolong teas produced in Fujian, the specific steps are as follows:

[0055] (1) Material preparation: 4 Tieguanyin samples are from Fujian Province, numbered FT1-FT4. The other two oolong tea samples also come from Fujian, namely Huang Jin Gui and Da Hong Pao, numbered FO1-FO2, both produced in 2013.

[0056] (2) Sample preparation: The PDMS / DVB / CAR extraction fiber head was aged at 250°C for 60 min at the GC inlet. Accurately weigh 2g of tea powder for each tea sample, put it into a 20mL headspace bottle, add 5mL of boiling distilled water for brewing, immediately seal the bottle mouth, and brew for 2 minutes. Then place it in the incubator of the headspace sampler at a speed of 250r / min. After equilibrating at 80°C for 10min, the autosam...

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Abstract

The invention discloses a method for differentiating oolong tea with different producing areas and fermentation types. Fragrance components in tea are extracted by a fully-automatic headspace solid-phase microextraction (HS-SPME) method, the fragrance components of the tea are separated and identified by combining gas chromatography-mass spectrometry (GC-MS), and a clustering and main component model is constructed by utilizing the relative content of the identified fragrance components of the tea and combining a chemometrics method (CA and PCA), so that the oolong tea with different producingareas and different fermentation types can be differentiated. The invention provides a new effective evaluation and control method for examining and identifying the quality of the tea (particularly producing area and fermentation type judgment based on the fragrance components). The method is simple and practical to operate, low in cost and environmentally friendly; and an efficient, objective and normalized method can be provided for performing quality evaluation and control on the tea.

Description

technical field [0001] The invention belongs to the technical field of tea, and in particular relates to a method for distinguishing oolong teas from different production areas and fermentation types by using full-automatic headspace solid-phase microextraction combined with gas chromatography mass spectrometry and chemometric methods. Background technique [0002] Oolong tea belongs to semi-fermented tea and is one of the six major teas in my country. Its main production areas are concentrated in northern Fujian (Wuyi rock tea, narcissus, Dahongpao, cinnamon, etc.), southern Fujian (Tieguanyin, Qilan, narcissus, golden osmanthus, etc.) ), Guangdong (Fenghuang Dancong, Fenghuang Narcissus, Lingtou Dancong, etc.) and Taiwan (Dongding Oolong, Baozhong, Oolong, etc.), the degree of fermentation is different in different regions. For example, Tieguanyin belongs to lightly fermented oolong tea , while Fenghuang Dancong and Dongfang Meiren are heavily fermented oolong teas. Oolong ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 孟庆雄
Owner 云南清莲生物科技有限公司
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