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Compound microbial liquid inoculant and application thereof

A technology of composite microorganisms and liquid bacterial agents, applied in the field of microorganisms, can solve problems such as the great influence of soil pH and organic matter richness, the obvious effect of microbial agents, and the difficulty of microbial survival, so as to improve the utilization rate of fertilizers, reduce moss, stable effect

Inactive Publication Date: 2019-01-04
郭春灵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microbial agents promoted in the market are single or compound agents, which have a single effect and unstable effects, and the application to the soil is also greatly affected by the pH of the soil and the richness of organic matter.
If the soil conditions are suitable, the effect of the microbial agent is obvious; on the contrary, the microorganisms themselves have difficulty in surviving, and it is difficult to play the expected role

Method used

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  • Compound microbial liquid inoculant and application thereof
  • Compound microbial liquid inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The freeze-dried powder of original strains (Bacillus subtilis, Lactobacillus rhamnosus, yeast and photosynthetic bacteria) was purchased from the strain bank of the Ministry of Agriculture of China, and the strains were recovered and expanded for cultivation.

[0034]Specific operations for expanded cultivation: medium sterilization (add 1500L pure water to a 2000L tank, add the fermenter medium into the tank while stirring when the temperature rises above 50°C, stir well, then start sterilization, steam heating Raise the temperature to 118°C and sterilize for 30 minutes. After the sterilization, take a sample from the sampling port to detect the pH value of the medium. Adjust the pH value to below 6.0, inoculate the sterile area (use water circulation to cool down, and when the tank temperature drops to 35°C, use flame Inoculation method inoculation), anaerobic fermentation (cultivation temperature is 35-37°C, tank pressure is kept at 0.05Mpa, stirring is started every...

Embodiment 2

[0042] Weigh 1860kg of purified water, 32kg of brown sugar, 2kg of peptone, 4kg of glucose, 2kg of yeast extract, and 100kg of composite bacteria described in Example 1. Mix to obtain the compound microorganism liquid inoculant.

Embodiment 3

[0044] Weigh 1766kg of purified water, 28kg of brown sugar, 1.5kg of peptone, 3kg of glucose, 1.5kg of yeast extract, and 100kg of compound bacteria described in Example 1. Mix to obtain the compound microorganism liquid inoculant.

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Abstract

The invention provides a compound microbial liquid inoculant and an application thereof, and belongs to the technical field of microorganisms. The compound microbial liquid inoculant comprises components in parts by mass as follows: 24-40 parts of brown sugar, 2-6 parts of glucose, 1-3 parts of peptone, 1-3 parts of yeast extract, 75-125 parts of compound bacteria and 1500-2200 parts of water, wherein the compound bacteria comprise Lactobacillus rhamnosus, Bacillus subtilis, saccharomyces and photosynthetic bacteria, and total quantity of effective viable bacteria in the compound bacteria is larger than or equal to 1*10<8> CFU / g. The compound microbial liquid inoculant is not affected by soil environment, the compound bacteria can propagate rapidly by nutrients of the inoculant and occupyecological niche in soil, and reproduction of harmful bacteria is reduced greatly.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial liquid bacterial agent and its application. Background technique [0002] In my country, scientific fertilization cannot be realized in many remote areas. For example, the long-term use of a large amount of immature chicken manure and the long-term single use of chemical fertilizers are likely to cause damage to the soil environment and cause soil problems such as compaction, salinization, and acid-base imbalance. Due to the problems in the soil, it is easy to cause the crops to take root but not to support the roots, and the root system will die after entering the soil, and cannot survive well. Studies have shown that crop diseases such as yellow leaves, small leaves, rotten roots, dead trees, root rot, root-knot nematodes, and fruit stains are all related to soil problems. [0003] Over the years, microbial agents have been strongly supp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C09K17/14A01N63/04A01N63/00A01N61/00A01N43/16A01P21/00C12R1/225C12R1/125C09K101/00
CPCA01N43/16A01N61/00A01N63/00A01N63/30C09K17/14C09K2101/00C12N1/16C12N1/20
Inventor 郭春灵张拥军张月鹏刘飞宇
Owner 郭春灵
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