Compound microbial liquid inoculant and application thereof
A technology of composite microorganisms and liquid bacterial agents, applied in the field of microorganisms, can solve problems such as the great influence of soil pH and organic matter richness, the obvious effect of microbial agents, and the difficulty of microbial survival, so as to improve the utilization rate of fertilizers, reduce moss, stable effect
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Embodiment 1
[0033] The freeze-dried powder of original strains (Bacillus subtilis, Lactobacillus rhamnosus, yeast and photosynthetic bacteria) was purchased from the strain bank of the Ministry of Agriculture of China, and the strains were recovered and expanded for cultivation.
[0034]Specific operations for expanded cultivation: medium sterilization (add 1500L pure water to a 2000L tank, add the fermenter medium into the tank while stirring when the temperature rises above 50°C, stir well, then start sterilization, steam heating Raise the temperature to 118°C and sterilize for 30 minutes. After the sterilization, take a sample from the sampling port to detect the pH value of the medium. Adjust the pH value to below 6.0, inoculate the sterile area (use water circulation to cool down, and when the tank temperature drops to 35°C, use flame Inoculation method inoculation), anaerobic fermentation (cultivation temperature is 35-37°C, tank pressure is kept at 0.05Mpa, stirring is started every...
Embodiment 2
[0042] Weigh 1860kg of purified water, 32kg of brown sugar, 2kg of peptone, 4kg of glucose, 2kg of yeast extract, and 100kg of composite bacteria described in Example 1. Mix to obtain the compound microorganism liquid inoculant.
Embodiment 3
[0044] Weigh 1766kg of purified water, 28kg of brown sugar, 1.5kg of peptone, 3kg of glucose, 1.5kg of yeast extract, and 100kg of compound bacteria described in Example 1. Mix to obtain the compound microorganism liquid inoculant.
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