Compost decomposition additive and application thereof
An additive and composting technology, which is applied in the direction of application, nitrogen fertilizer, phosphate fertilizer, etc., can solve the problems of perishable rancidity, high water content, environmental pollution, etc., and achieve the effect of convenient compost fermentation, reducing the amount of use, and promoting reproduction
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Embodiment 1
[0018] Aspergillus niger was inoculated in PDA medium, cultured at 30°C at 220-250r / min for 72 hours, and the number of spores reached 2-2.5×10 8 CFU / ml, as mature seed solution.
[0019] Penicillium was inoculated in PDA medium, cultured at 30°C at 220-250r / min for 72 hours, and the number of spores reached 2-2.5×10 8 CFU / ml, as mature seed solution.
[0020] Ralstonia sp. (Shanghai Kewei Chemical Technology Co., Ltd.) medium: peptone 0.5%, beef extract 0.3%, magnesium sulfate 0.5-1%, NaCl 0.5%, agar 1.5%, pH7 .0, 220~250r / min culture for 48-72 hours, the bacteria concentration reached 1×10 8 CFU / ml, as mature seed solution.
[0021] Medium for Candida utilis (purchased from China Industrial Microorganism Culture Collection Center): 2-3% peptone, 5% glucose, 0.5-1% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.5% ammonium sulfate, pH 5.5, cultured at 220-250r / min, 12-24 hours, the bacterial concentration reached 1.5-2×10 8 CFU / ml, as mature seed solution.
Embodiment 2
[0023] Add 100 grams of bran in every 1000 grams of fresh brewer's grains, 30 grams of ammonium sulfate, 15 grams of urea, 10 grams of potassium dihydrogen phosphate, and 5 grams of NaCl; 15% of the mass (w) is sequentially inoculated with the above-mentioned seed solution, wherein Aspergillus niger 45ml, Penicillium 45ml, Ralstonia 30ml and Candida utilis 30ml; fermentation 48-72 hours.
Embodiment 3
[0025] Add 50 grams of bran, 20 grams of ammonium sulfate, 10 grams of urea, 8 grams of potassium dihydrogen phosphate, and 3 grams of NaCl in every 1000 grams of the quality of fresh brewer's grains; 18% of the mass (w) is sequentially inoculated with the above-mentioned seed liquid, wherein Aspergillus niger 40ml, Penicillium 40ml, Ralstonia 20ml and Candida utilis 20ml; ferment for 48-72 hours.
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