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Preparation method of anti-liver cancer nanoparticles loaded with two drugs and having double-layer controlled release-magnetic targeting-photothermal-magnetic thermal function

A nanoparticle and magnetic targeting technology, applied in the field of preparation of anti-hepatoma nanoparticles, can solve the problems of patient trauma, complications, normal cell damage, etc., and achieve the effects of prolonging drug action time, improving packaging efficiency, and improving stability.

Active Publication Date: 2020-12-18
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And these methods all have great risks, which are likely to bring great trauma and complications to patients, and at the same time, they are likely to cause greater damage to normal cells while killing cancer cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]1) Add 1 g of ferroferric oxide nanoparticles to 100 mL of deionized water, and ultrasonically disperse for 1 min;

[0040]2) Put the solution in step 1) in a water bath and raise the temperature to 60 ℃, stir and react for 1 h under the protection of nitrogen, and use sodium hydroxide solution (0.008 mol·L-1) Maintain the pH of the solution at 9;

[0041]3) Then add 5% dilute hydrochloric acid to adjust the solution in step 2) to neutrality, take 0.9mL 3-aminopropyltriethoxysilane and add it to the dispersion, stir for 2 h, add 9mL ammonia water dropwise After continuing the reaction for 1 h, let it stand for 6 h, filter with suction, wash the particles with deionized water 3 times, and dry at 60 ℃ for 12 h;

[0042] 4) Dissolve 2 g of carboxymethyl chitosan in 100 mL of deionized water, and then add 1 g of the aminated ferroferric oxide particles in step 3) to the solution. After sonicating for 1 min, add to the mixture while stirring 1.5g of 1-(3-dimethylaminopropyl)-3-ethylcarbodi...

Embodiment 2

[0054]1) Take 1.2 g of Fe3O4 nanoparticles into 130 mL of deionized water, and ultrasonically disperse for 1.5 min;

[0055]2) Put the solution in step 1) in a water bath and raise the temperature to 60 ℃, stir and react for 1.2 h under the protection of nitrogen, and use sodium hydroxide solution (0.008 mol·L-1) Maintain the pH of the solution at 9;

[0056]3) Then add 5% dilute hydrochloric acid to adjust the solution in step 2) to neutrality, add 1 mL of 3-aminopropyltriethoxysilane to the dispersion, stir for 2.5 h, and add 10 mL of ammonia dropwise. After continuing the reaction for 1.5 h, let it stand for 6 h, filter with suction, wash the particles with deionized water 3 times, and dry at 60 ℃ for 12 h;

[0057]4) Dissolve 2.5 g of carboxymethyl chitosan in 125 mL of deionized water, and then add 1.5 g of the aminated ferroferric oxide particles in step 3) to the solution. After sonicating for 1.5 min, add to the mixture while stirring 1.7 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodi...

Embodiment 3

[0069]1) Take 1.5 g of ferroferric oxide nanoparticles and add them to 150 mL of deionized water, and ultrasonically disperse for 2 min;

[0070]2) Put the solution in step 1) in a water bath and raise the temperature to 60 ℃, and stir the reaction for 1.5 h under the protection of nitrogen. During this time, use sodium hydroxide solution (0.008 mol·L-1) Maintain the pH of the solution at 10;

[0071]3) After adding 5% dilute hydrochloric acid to adjust the solution in step 2) to neutrality, add 1.2 mL of 3-aminopropyltriethoxysilane to the dispersion, stir for 3 h, and add 12 mL of ammonia dropwise After continuing the reaction for 1-2 h, let it stand for 6 h, filter with suction, wash the particles with deionized water 4 times, and dry at 60 ℃ for 12 h;

[0072]4) Dissolve 3 g of carboxymethyl chitosan in 150 mL of deionized water, and then add 2 g of the aminated ferroferric oxide particles in step 3) to the solution. After sonicating for 2 minutes, add to the mixture while stirring 2 g o...

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Abstract

The invention relates to the field of pharmacy and discloses a method for preparing two-medicine-loaded anti-hepatoma nanoparticles with double-layer controlled release, magnetic targeting, photo-thermal and magneto-thermal functions. The method includes: modifying ferroferric oxide on carboxymethyl chitosan to achieve a targeting function and a magneto-thermal function; adopting polydopamine forloading an anti-cancer medicine namely doxorubicin, and embedding into hydrophilic polyvinylpyrrolidone and Arabic gum; loading an anti-cancer medicine namely Sorafenib on the oil-phase outer surfaceof hydrophilic polyvinylpyrrolidone and Arabic gum; finally, embedding the above system through carboxymethyl chitosan-ferroferric oxide nanoparticle aqueous solution to obtain magnetic-targeted double-layer synergistic controlled release nanoparticles for joint treatment of hepatoma through two anti-cancer medicines and photo-thermal and magneto-thermal therapy. The polymeric particles can realize controlled release of double anti-cancer medicines, and cancer cells are treated under the joint action of the magneto-thermal property of ferroferric oxide and the photo-thermal property of polydopamine.

Description

Technical field[0001]The present invention relates to the field of pharmacy, in particular to a method for preparing anti-liver cancer nano particles loaded with two drugs and having the functions of double-layer controlled release-magnetic targeting-photothermal-magnetothermal.Background technique[0002]As a major disease threatening human life and health, cancer has long been characterized by low cure rate, high recurrence and high mortality. Liver cancer is also one of the most common malignant tumors in my country. Surgery, radiotherapy, and chemotherapy are currently the main methods and methods of cancer treatment. These methods have great risks and are likely to cause major trauma and complications to patients. At the same time, they can kill cancer cells and cause greater damage to normal cells. In order to improve the efficacy of drugs and reduce the side effects of drugs, there has been a scheme of using drug carriers to deliver drugs. The drug is wrapped by chemical bondin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K31/704A61K47/58A61K47/36A61K47/59A61K41/00A61P35/00A61K31/44
CPCA61K9/0009A61K9/5161A61K31/44A61K31/704A61K41/0052A61K47/58A61K47/59A61P35/00A61K2300/00
Inventor 王秉金小康陈碧玲万军民胡智文
Owner ZHEJIANG SCI-TECH UNIV
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