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Method for preparing adipose-derived stem cell sap for cosmetic maintenance

A technology of fat stem cells and cells, which is applied in the field of medical cosmetology, can solve the problems of not being able to effectively promote the metabolism of epidermal cells, having no biologically active substances, and many harmful substances remaining, so as to promote skin wound healing, prolong storage time, and prevent discoloration. spot effect

Inactive Publication Date: 2018-12-28
丁彬彬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the existing beauty care products are made by chemical methods, and there are many harmful substances in the products, and long-term use will have adverse effects on the skin
Moreover, most of the existing products do not have biologically active substances, which cannot effectively promote the metabolism of epidermal cells and improve the luster of the skin.
Moreover, some substances contained in chemical skin care products will cause allergic reactions to the human body, so they are not suitable for all people.

Method used

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preparation example Construction

[0025] The embodiment of the present invention provides a preparation method of adipose stem cell fluid for beauty maintenance, comprising the following steps:

[0026] Step 1: Obtain adipose stem cells, obtain adipose tissue, wash with D-Hanks buffer to remove residual blood and tissue fragments, cut the adipose tissue into small fat pieces, add the adipose tissue to the digestive juice, and put it in a constant temperature shaking box at 37°C Digest in medium for 20-60 minutes, then let it stand until it is stratified, absorb the upper fat cell liquid, transfer it into the DMEM medium containing fetal bovine serum, seal and centrifuge, the speed of centrifugation is 800rpm-1800rpm, and the centrifugation time is 5min- After centrifugation for 15 minutes, remove the supernatant, add an appropriate amount of DMEM medium containing fetal bovine serum again, pipette the cells to make adipose-derived stem cell suspension, inoculate into culture flasks, and store at 37°C in 5% CO ...

Embodiment 1

[0034] Prepare adipose-derived stem cell fluid by the following steps:

[0035] Step 1: Obtain adipose stem cells. First, obtain adipose tissue. The adipose tissue can be obtained from the human body through liposuction. The adipose tissue is washed with D-Hanks buffer to remove residual blood and tissue fragments, and the adipose tissue is cut into For small fat pieces, add the fat tissue into the digestion solution, put it in a 37°C constant temperature shaking box for digestion for 20 minutes, then let it stand until it is layered, absorb the upper fat cell liquid, transfer it into the DMEM medium containing fetal bovine serum, seal and centrifuge Separation, the speed of centrifugation is 800rpm, and the centrifugation time is 15min. After centrifugation, the supernatant is removed, and an appropriate amount of DMEM medium containing fetal bovine serum is added again. At 37°C, 5% CO 2 Culture at a constant temperature, and replace the medium regularly until the adipose st...

Embodiment 2

[0040] Prepare adipose-derived stem cell fluid by the following steps:

[0041] Step 1, to obtain adipose stem cells, first obtain adipose tissue, wash the adipose tissue with D-Hanks buffer to remove residual blood and tissue fragments, cut the adipose tissue into small fat pieces, add the adipose tissue to the digestive juice, and put it in 37℃ Digest in a constant temperature shaking box for 30 minutes, then let it stand until it is layered, absorb the upper fat cell liquid, transfer it into the DMEM medium containing fetal bovine serum, seal and centrifuge, the speed of centrifugation is 800rpm, and the centrifugation time is 15min. Remove the supernatant after separation, add an appropriate amount of DMEM medium containing fetal bovine serum again, pipette the cells to make adipose stem cell suspension, inoculate into culture flasks, and store at 37°C, 5% CO 2 Culture at a constant temperature, and replace the medium regularly until the adipose stem cells cover the bottom...

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Abstract

The invention relates to a method for preparing adipose-derived stem cell sap for cosmetic maintenance and belongs to the technical field of medical beauty. The method includes step 1 of obtaining adipose-derived stem cells; step 2 of subculture; step 3 of low temperature freezing; and step 4 of preparation of the adipose-derived stem cell sap. The method for preparing the adipose-derived stem cell sap is simple and convenient to operate, the production technology requirements are not high, the method is suitable for large-scale production of cell sap products, the production process can be developed for a long time in a large scale in a biotechnology production workshop, the manufacturing cost is low, the product effect is very good, the retention time of the adipose-derived stem cell sapcan be prolonged through the low temperature freezing step, a frozen state is removed when the adipose-derived stem cell sap needs to be used, and the market prospect is broad.

Description

technical field [0001] The invention relates to a method for preparing adipose stem cell fluid for beauty maintenance, and belongs to the technical field of medical cosmetology. Background technique [0002] In the modern society where the life expectancy of human beings continues to prolong and the population is aging day by day, aging and longevity issues have attracted much attention from all sides, and slowing down the aging of the skin has gradually become the goal pursued by people. The aging of the human body and the appearance of wrinkles are essentially the aging and reduction of cells. The senescence and reduction of cells is caused by the aging of stem cells. [0003] Adipose-derived stem cells (ADSCs) and ADSC pluripotent cells are a kind of stem cells with multipotential differentiation potential isolated from adipose tissue in recent years. It mainly restores the repair function of tissue cells, promotes cell regeneration, restores a youthful appearance, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02A61K8/99A61K8/64A61Q19/02A61Q19/08
CPCA01N1/0221A61K8/64A61K8/99A61Q19/02A61Q19/08C12N5/0667
Inventor 丁彬彬
Owner 丁彬彬
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