Fixed-bed bioreactor with constant-flow pump/tubing system

A bioreactor, constant rate technology, applied in specific-purpose bioreactors/fermenters, methods of supporting/immobilizing microorganisms, bioreactor/fermenter combinations, etc., can solve problems such as destroying pump work

Pending Publication Date: 2018-12-21
TRIZELL LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, unlike liquid media, air bubbles expand in this negative pressure environme...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fixed-bed bioreactor with constant-flow pump/tubing system
  • Fixed-bed bioreactor with constant-flow pump/tubing system
  • Fixed-bed bioreactor with constant-flow pump/tubing system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0038] Example 3 - Unidirectional Sampling Manifold

[0039] Bioreactor sampling manifolds are used to obtain liquid samples of cell culture medium from the bioreactor vessel, for example to check pH or to measure glucose, metabolites and cellular waste products (e.g. lactate) in the medium or other substances.

[0040] We unexpectedly found that, as commercially available iCELLis TM The stock sampling manifold supplied as part of the ® is susceptible to contamination. We successfully designed a new sampling manifold for the bioreactor to eliminate the need for pumping media into and out of the bioreactor vessel into sampling bottles demand. In this way we minimize the risk of contamination by sampling.

[0041] A schematic diagram of the adherent culture bioreactor is shown in figure 2 middle. The bioreactor comprises a vessel [1] containing a cell culture plate [2] to which cells adhere when cultured in an adherent mode. The container [1] contains liquid cell cultu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.

Description

[0001] related application [0002] This application claims priority to US Provisional Patent Application Serial No. 62 / 322651 filed April 14, 2016, the contents of which are incorporated herein by reference. [0003] government interest [0004] none. Background technique [0005] We have improved commercial scale adherent cell culture by developing an improved bioreactor that provides -50% increase in productivity compared to prior art bioreactors while also eliminating sources of contamination . [0006] Commercially available adherent cell culture bioreactors include, for example, the lab-scale iCELLis TM Nano (commercially available from Pall Corporation, Cambridge Masachusetts) and commercial scale iCELLis TM 500 bioreactor, the latter provides cell culture medium volumes up to 65 liters and cell culture plates of pharmaceutical grade polyester microfibers that provide up to 500 m 2 growth area. We improved the functionality of this commercial-scale bioreactor by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/00C07K1/14C12M1/00
CPCC12M1/02C12P21/02C12M25/18C12M29/10C12N15/86C12N2740/15051C12M29/18C12M41/26C12M41/46C07K14/005
Inventor 汉娜·P·莱施皮亚·克里斯蒂娜·瓦洛宁艾娃·克里斯蒂娜·雷塞宁特尔佳·汉内莱·图纳宁米利亚乔娜·卡罗利娜·卡尔纳图阿希姆·米勒米娜·克里斯蒂娜·卡尔希宁
Owner TRIZELL LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap