Fixed-bed bioreactor with constant-flow pump/tubing system

Abstract

We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.

Description

[0001] related application [0002] This application claims priority to US Provisional Patent Application Serial No. 62 / 322651 filed April 14, 2016, the contents of which are incorporated herein by reference. [0003] government interest [0004] none. Background technique [0005] We have improved commercial scale adherent cell culture by developing an improved bioreactor that provides -50% increase in productivity compared to prior art bioreactors while also eliminating sources of contamination . [0006] Commercially available adherent cell culture bioreactors include, for example, the lab-scale iCELLis TM Nano (commercially available from Pall Corporation, Cambridge Masachusetts) and commercial scale iCELLis TM 500 bioreactor, the latter provides cell culture medium volumes up to 65 liters and cell culture plates of pharmaceutical grade polyester microfibers that provide up to 500 m 2 growth area. We improved the functionality of this commercial-scale bioreactor by...

AI Technical Summary

Problems solved by technology

However, unlike liquid media, air bubbles expand in this negative pressure environment, absorbing a large amount of negative pressure energy, thereby disrupting pump operation

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