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Simple and easy trace cell culture and counting device and manufacturing method of device

A technology of micro-cells and production methods, which can be used in tissue cell/virus culture devices, biochemical equipment and methods, enzymology/microbiology devices, etc. It can solve the problems of high instrument cost, large sample volume, and complicated operation. Achieve the effect of high biocompatibility, good air permeability and rapid counting

Inactive Publication Date: 2018-12-21
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flow cytometry can accurately count micro-cells, but the cost of the instrument is high, the operation is complicated, and the sample volume required is large
And the existing cell counting device usually only has the counting function, and the function is relatively single

Method used

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  • Simple and easy trace cell culture and counting device and manufacturing method of device
  • Simple and easy trace cell culture and counting device and manufacturing method of device
  • Simple and easy trace cell culture and counting device and manufacturing method of device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Counting of circulating tumor cells in whole blood of patients with breast cancer

[0038] Since the number of circulating tumor cells in patients with solid tumors is very small, there may be only a few tumor cells in 1mL of whole blood. How to accurately count the captured tumor cells is one of the urgent problems to be solved.

[0039] The present invention designs a device that can accurately count microcells, and its structure is as attached figure 1 As shown, the device of the present invention includes a base plate 1 and a base plate 2; the base plate is arranged on the upper end of the base plate 1, and the base plate 1 is a common glass slide, which is beneficial to loading and culturing cells; the base plate 2 is made of PDMS material, and the base plate 2 There are several through-holes 21 penetrating up and down, and the aperture size of the through-holes is 4 mm, matching with the 5× objective lens on the microscope.

[0040] The device is made a...

Embodiment 2

[0046] Accurate counting of embodiment 2CCRF-CEM cells

[0047] The cell counting device of the present invention can also accurately count a relatively large number of cells, and has the advantages of low cost, easy operation and high accuracy. The structure and manufacturing method of the cell counting device in this embodiment are the same as those in Embodiment 1, except that the substrate 2 is made of PMMA material. The CCRF-CEM cells in the culture dish were incubated with DIO solution at 37°C for 10 min, so that the cells had green fluorescence under 488nm excitation. Redisperse the cells in 20 μL PBS, the concentration range is 1-1000 / mL (over this concentration range, the cells are easy to superimpose to form two layers, and the accurate count of cells cannot be carried out at this time), dropwise on one of the inventive devices In the well, let it stand for 5 minutes, and take pictures with a fluorescence microscope (5× objective lens). The acquired pictures are proce...

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Abstract

The invention discloses a simple and easy trace cell culture and counting device and a manufacturing method of the device. The device comprises a bottom plate and a base plate, wherein the base plateis arranged at the upper end of the bottom plate; the base plate is tightly combined with the bottom plate; the bottom plate is a glass culture dish; the base plate is made of a material with high transparency and good biocompatibility; the base plate is provided with a plurality of through holes which are through up and down; the pore diameter size of each through hole is matched with the size ofa view field needed in a microscope. Compared with an existing cell counting device, the device is low in cost and can be repeatedly used for a plurality of times; the utilized material has good airpermeability, high biocompatibility, strong elasticity and no damages to cells; cell culture is facilitated and cell culturing and cell counting functions can be integrated; the accuracy is high and the device is suitable for counting trace cells; the device is rapid in counting speed and a plurality of samples can be counted on one base plate at the same time.

Description

technical field [0001] The invention relates to the technical field, in particular to a simple micro-cell culture and counting device and a manufacturing method of the device. Background technique [0002] In biological research, it is sometimes necessary to measure the number of cells per unit volume. In the existing counting process, a cell counting board is generally used to measure the number of cells. The existing device for cell counting usually uses a hemocytometer Counting plate, flow cytometry; hemocytometer is used for microscopic counting of red and white blood cells in the human body, and is also commonly used to count the number of some bacteria, fungi, yeast and other microorganisms. It is a common biological tool ; Patent CN206696154U discloses a blood cell counting board, including a base plate and a cover sheet, the upper surface of the base plate is provided with a counting pool, and the base plate is provided with a movable liquid separator, and the blood ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12M1/22
CPCC12M23/20C12M41/36
Inventor 陈永丽张珝
Owner SHENZHEN POLYTECHNIC
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