A kind of construction method of animal model of epilepsy
A technology for epilepsy animals and mice, applied in the fields of biochemical equipment and methods, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of improving the pathogenesis of epilepsy and having a long life cycle
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[0031] Example 1 Method for Conditional Knockout of FGF9 Gene in GABAergic Neurons and Construction of Epilepsy Animal Model
[0032] First, FGF9 fl / fl The mouse preparation steps are as follows:
[0033] 1) Construction of a targeting vector for conditional knockout of FGF9
[0034] Analyze the structure of the FGF9 gene, exon1 (ENSMUSE00000337574) can be conditionally knocked out, the FGF9 gene is not expressed after knockout, and the loxP sites at the 5' end and 3' end are inserted in the promoter region and the non-conserved region of intron2, respectively;
[0035] 2) Electroporation of ES cells and screening of ES cell clones with correct recombination
[0036] After the targeting vector was linearized by restriction enzyme digestion, the ES cells were electroporated twice, and a total of 600 clones were selected. After screening by PCR and Southern blot, a total of 10 ES cell clones with correct recombination at both ends were screened;
[0037] 3) Microinjection of ES...
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