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A kind of construction method of animal model of epilepsy

A technology for epilepsy animals and mice, applied in the fields of biochemical equipment and methods, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of improving the pathogenesis of epilepsy and having a long life cycle

Active Publication Date: 2021-09-07
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But up to now, there is no report on the relationship between FGF9 and epilepsy at home and abroad

Method used

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  • A kind of construction method of animal model of epilepsy
  • A kind of construction method of animal model of epilepsy
  • A kind of construction method of animal model of epilepsy

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Method for Conditional Knockout of FGF9 Gene in GABAergic Neurons and Construction of Epilepsy Animal Model

[0032] First, FGF9 fl / fl The mouse preparation steps are as follows:

[0033] 1) Construction of a targeting vector for conditional knockout of FGF9

[0034] Analyze the structure of the FGF9 gene, exon1 (ENSMUSE00000337574) can be conditionally knocked out, the FGF9 gene is not expressed after knockout, and the loxP sites at the 5' end and 3' end are inserted in the promoter region and the non-conserved region of intron2, respectively;

[0035] 2) Electroporation of ES cells and screening of ES cell clones with correct recombination

[0036] After the targeting vector was linearized by restriction enzyme digestion, the ES cells were electroporated twice, and a total of 600 clones were selected. After screening by PCR and Southern blot, a total of 10 ES cell clones with correct recombination at both ends were screened;

[0037] 3) Microinjection of ES...

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Abstract

The invention relates to a method for constructing an animal model of epilepsy, and belongs to the technical field of animal model construction. This method converts FGF9 fl / fl Mice mated with GABAergic neuronal Cre mice to acquire FGF9 fl / + VGAT‑Cre heterozygous knockout mice, followed by FGF9 fl / fl Homozygous mice were mated to obtain FGF9 homozygous knockout mice (FGF9 fl / fl VGAT‑Cre), an animal model of epilepsy. This epilepsy animal model is closer to clinical epilepsy symptoms and mechanisms, and provides a good research tool for the study of clinical epilepsy etiology, as well as further diagnosis and treatment.

Description

technical field [0001] The invention relates to a method for constructing an animal model, and more particularly relates to a method for constructing an animal model of epilepsy, which belongs to the technical field of animal model construction. Background technique [0002] Epilepsy is a group of chronic diseases and syndromes of sudden, short-lived and recurrent central nervous system (Central nervous system, CNS) dysfunction caused by abnormal excessive discharge of brain neurons. One of the neurological diseases. According to the estimates of the World Health Organization, there are more than 70 million epilepsy patients in the world, accounting for about 1% of the world's population. The prevalence of epilepsy in my country is between 4% and 7%. There are about 6 million active epilepsy patients, and about 400,000 new epilepsy patients each year. Epilepsy can seriously reduce the quality of life of patients, increase the burden on patients' families and society, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85A01K67/027
CPCA01K67/027A01K2217/075A01K2227/105A01K2267/0356C07K14/50C12N15/8509C12N2800/107C12N2800/30
Inventor 李春岩郭默然段伟松伊乐李忠尧刘亚坤毕悦李媛媛张杰
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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