Method for establishment of tumor lymph node high-metastasis model and rapid fluorescence detection
A technology of lymph node metastasis and detection method, which is applied in the establishment of tumor lymph node high metastasis model and the field of rapid fluorescence detection, which can solve the problem of tumor recurrence in patients and achieve the effect of broad application prospects.
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[0049] The method for making a model of a rabbit neck highly metastatic lymph node model provided by the invention comprises the following steps:
[0050] Step S1: Obtain the VX2 tissue block of rabbit epithelial squamous cell carcinoma. The specific steps are: purchase the VX2 tumor-bearing rabbit, and after about 25 days of the growth of the leg tumor, aseptically excise the VX2 tumor tissue block of the leg of the tumor-bearing rabbit, and select the Fish-like tumor tissue, chopped to a volume of about 0.5mm 3 , placed in saline.
[0051] Step S2: Establish a rabbit tongue cancer model. The specific steps are as follows: take two healthy New Zealand white rabbits, place them on a fixed frame, inject 3mL of 2.5% pentobarbital sodium through the ear vein, and after the anesthesia is successful, use a 1mL syringe to inject the VX2 tumor tissue described in step S1 The block is drawn into the syringe. Install a 9-gauge needle on a 1mL syringe, and inject the tissue block int...
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