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Method for establishment of tumor lymph node high-metastasis model and rapid fluorescence detection

A technology of lymph node metastasis and detection method, which is applied in the establishment of tumor lymph node high metastasis model and the field of rapid fluorescence detection, which can solve the problem of tumor recurrence in patients and achieve the effect of broad application prospects.

Inactive Publication Date: 2018-12-18
NANJING UNIV
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  • Abstract
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Problems solved by technology

Although the primary tumor can be controlled by means of surgery, radiotherapy and chemotherapy, some patients still die of tumor recurrence

Method used

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  • Method for establishment of tumor lymph node high-metastasis model and rapid fluorescence detection
  • Method for establishment of tumor lymph node high-metastasis model and rapid fluorescence detection
  • Method for establishment of tumor lymph node high-metastasis model and rapid fluorescence detection

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Embodiment Construction

[0049] The method for making a model of a rabbit neck highly metastatic lymph node model provided by the invention comprises the following steps:

[0050] Step S1: Obtain the VX2 tissue block of rabbit epithelial squamous cell carcinoma. The specific steps are: purchase the VX2 tumor-bearing rabbit, and after about 25 days of the growth of the leg tumor, aseptically excise the VX2 tumor tissue block of the leg of the tumor-bearing rabbit, and select the Fish-like tumor tissue, chopped to a volume of about 0.5mm 3 , placed in saline.

[0051] Step S2: Establish a rabbit tongue cancer model. The specific steps are as follows: take two healthy New Zealand white rabbits, place them on a fixed frame, inject 3mL of 2.5% pentobarbital sodium through the ear vein, and after the anesthesia is successful, use a 1mL syringe to inject the VX2 tumor tissue described in step S1 The block is drawn into the syringe. Install a 9-gauge needle on a 1mL syringe, and inject the tissue block int...

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Abstract

The invention discloses a method for establishing a model of a large animal tumor lymph node high-metastasis model and rapidly detecting the lymph node containing tumor cells in real time. The methodcomprises the following steps: establishing a rabbit tongue tumor model, and establishing a rabbit cervical lymph node high-metastasis model based on the rabbit tongue tumor model; after successful establishment of the rabbit cervical high-metastasis model, an endogenous substance 5-aminolevulinic acid (5-ALA) is used, and the fluorescence of its metabolite protoporphyrin (PPIX) is used to rapidlyand accurately detect the rabbit cervical lymph node. The method has the advantages of simple operation and high reliability, fully and completely simulates the clinical characteristics and biological characteristics of human tongue cancer lymph node metastasis, provides a research model for the treatment and resection of lymph node metastasis of human tumors, provides a novel means for intraoperative visualization of real-time tumor lymph node dissection, and has broad application prospects in the fields of biomedical and photodynamic diagnosis.

Description

technical field [0001] The invention belongs to the fields of biomedicine and optics, and relates to the establishment of animal models and the photodynamic diagnosis of diseases, in particular to the establishment of a tumor lymph node high metastasis model and a rapid fluorescence detection method. Background technique [0002] Today, with the high development of modern medical care, tumors, as a disease that has not been completely conquered, have caused 2.81 million deaths per year in China (the latest cancer data in China in 2017. Chinese Cancer Clinic and Rehabilitation, 2017, 24(6) :760.). Although the primary tumor can be controlled by means of surgery, radiotherapy and chemotherapy, some patients still die of tumor recurrence. Lymph node metastasis is the primary cause of tumor recurrence: the infiltrating tumor cells pass through the wall of the lymphatic vessel, fall off and reach the surrounding lymph nodes with the lymph fluid, where they develop into new tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K49/00
CPCA61K49/0021A61K35/12
Inventor 王毅庆张旭东卢乾谢曼曼王子阳倪轲娜何忆霖韩久园
Owner NANJING UNIV
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