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A method for improving the seed setting rate of cross pollination of white konjac and flower konjac

A technology of cross-pollination and flower konjac, which is applied in the direction of botany equipment and methods, plant gene improvement, application, etc., can solve the problem of low seed setting rate, achieve high pollination and seed setting rate, increase storage time, and achieve the effect of accurate hybridization time

Active Publication Date: 2022-05-10
ANKANG AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seed setting rate of hybridization of white konjac pollen collected in the field and flower konjac is also low

Method used

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  • A method for improving the seed setting rate of cross pollination of white konjac and flower konjac
  • A method for improving the seed setting rate of cross pollination of white konjac and flower konjac

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0025] (1) Collection of white konjac pollen:

[0026] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28°C, the light intensity is 4950Lx, the humidity is 45%, and the culture time is 24h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0

[0027] (2) cryopr...

Embodiment 2

[0035] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0036] (1) Collection of white konjac pollen:

[0037] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28°C, the light intensity is 5000Lx, the humidity is 50%, and the culture time is 36h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0

[0038] (2) cryopr...

Embodiment 3

[0046] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0047] (1) Collection of white konjac pollen:

[0048] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28.5°C, the light intensity is 5050Lx, the humidity is 55%, and the culture time is 48h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0

[0049] (2) cryo...

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Abstract

The invention discloses a method for improving the hybrid pollination and seed setting rate of white konjac and flower konjac, which comprises the following steps: (1) collecting the pollen of white konjac by artificial pollination in an illumination incubator; (2) Preserve the collected white konjac pollen by cryopreservation; (3) revive the cryopreserved white konjac pollen; (4) select flower konjac with fully mature pistil and recovered white konjac pollen for artificial pollination and bagging treatment , when the color of the ovary starts to turn green, the bagging process can be removed. Compared with the prior art, the beneficial effect of the present application is that the amount of white konjac pollen, the purity of the pollen and the vigor of the pollen are all significantly higher than those collected naturally in the field; The rate of pollination and seed setting was significantly improved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for improving the hybrid pollination and seed setting rate of white konjac and flower konjac. Background technique [0002] Konjac is a perennial herbaceous plant of the Araceae Konjac genus. It is the only plant rich in glucomannan found so far in nature. Glucomannan has gelling, cohesive, film-forming, soluble, thickening and water-retaining properties. It is widely used in food, chemistry, medicine, environmental protection and other fields. Konjac germplasm resources are abundant, there are about 170 species in the world, and 21 species are endemic to my country. The existing cultivated varieties in China are mainly flower konjac, white konjac and bulbil konjac, and most of them are local population species, and the variety traits are uneven. In addition, The national konjac production areas are still dominated by unimproved original species, rathe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 段龙飞郭邦利覃剑锋蔡阳光陈国爱张百忍刘次鹏王宗方
Owner ANKANG AGRI SCI RES INST
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