A kind of culture method of iron oxidation leaching microorganism

A technology of ore leaching microorganisms and culture methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that iron-containing sulfate ions cannot be discharged directly, the cell concentration does not increase significantly, and the delay of ore leaching microorganisms is shortened period and other problems, to achieve the effect of easy solid-liquid separation, shorten the culture time, and reduce the loss

Active Publication Date: 2019-07-30
CENT SOUTH UNIV
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Problems solved by technology

However, the concentration process is driven by a high-pressure circulating pump. A large amount of heat and shear force formed by repeated concentration will cause damage to microbial cells and affect microbial activity. With the increase of cell concentration, the membrane is likely to cause membrane blockage. When the concentration of leaching microorganisms condensed to 10 12 It is difficult to further concentrate above cells / mL
The concentration process also produces a large amount of sterile waste liquid, and a large amount of iron, ammonia nitrogen, phosphate and sulfate ions cannot be discharged directly
[0005] In addition, there is a method for shortening the lagging period of ore leaching microorganisms. Patent application number CN201210322233.4 discloses a cell culture method for quickly starting the activity of iron-oxidizing bacteria. It is only for the purpose of improving the activity of the seed solution. The substrate in the liquid that may inhibit the growth of microorganisms is reduced to energy substances available to the bacteria, and the lag period after inoculation is shortened from the original 15-30 hours to 6-10 hours, which is suitable for the rapid start or expansion of iron-oxidizing bacteria culture Culture, although the cell viability can be improved, but the cell concentration in the solution does not increase significantly, and the time from the lag period to the complete oxidation of ferrous iron is still about 40-50 hours

Method used

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  • A kind of culture method of iron oxidation leaching microorganism
  • A kind of culture method of iron oxidation leaching microorganism

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1) Cultivate Acidithiobacillus ferrooxidans in a 5L aeration packing tank, the medium is a standard 9K medium, pH 1.8, the temperature is controlled at 30°C, microporous aeration is used, the aeration rate is 0.3vvm, the initial The microbial concentration is 1.2×10 7 cells / mL, cultured for 48 hours, the microbial concentration reached 1×10 8 cells / mL, oxidation-reduction potential 671mV, transfer the culture solution to a 5L conical-bottom stirred culture tank, stir, and add 30g of iron powder with a particle size of 200 mesh. After the iron powder is completely dissolved, the total iron ion concentration does not exceed 20g / mV L, then add sulfuric acid solution to adjust the pH to 1.8, continue to stir and aerate for 8 hours, the stirring speed is 60rpm, and the aeration rate is 0.3vvm;

[0046] 2) When adding sulfuric acid solution in step 1), when the oxidation-reduction potential of the culture solution after aerated culture is 650-716mV, transfer the culture solu...

Embodiment 2

[0050] 1) Cultivate active Leptospirillum ferriphilum in a 300L aerated packing tank, the medium is a standard 9K medium, the initial ferrous ion concentration is 9g / L, and the initial microbial concentration is 1.5×10 7 cells / mL, pH value 1.5, controlled temperature 45°C, microporous aeration, aeration rate 0.3vvm, cultured for 48 hours, the microbial concentration reached 2.4×10 8 cells / mL, oxidation-reduction potential 705mV, transfer the culture solution to a 300L conical bottom stirring culture tank, stir, and add 2.7Kg of iron powder with a particle size of 200 mesh, after the iron powder is completely dissolved, the total iron ion concentration should not exceed 20g / L, then add sulfuric acid solution to adjust the pH to 1.0, continue to stir and aerate for 7 hours, the stirring speed is 120rpm, and the aeration rate is 0.1vvm;

[0051] 2) When adding sulfuric acid solution in step 1), when the oxidation-reduction potential of the culture solution after aerated culture ...

Embodiment 3

[0055] 1) Cultivate the mixed bacteria of Leptospirillum ferriphilumYSK and Sulfobacillus thermosulfidooxidansYN22 in a 1000L aerated packing tank, the medium is standard 9K medium, and the initial ferrous ion concentration is 9g / L, add yeast 0.005%, initial microbial concentration 2×10 7 cells / mL, pH value 1.5, controlled temperature 45°C, microporous aeration, aeration rate 0.3vvm, cultured for 60 hours, the microbial concentration reached 2.4×108 cells / mL, oxidation-reduction potential 675mV, transfer the culture solution to a 1000L conical bottom stirring culture tank, stir, and add 11Kg of iron powder with a particle size of 200 mesh, after the iron powder is completely dissolved, the total iron ion concentration does not exceed 20g / L, then add sulfuric acid solution to adjust the pH to 2.0, continue to stir and aerate for 8 hours, the stirring speed is 120rpm, and the aeration rate is 0.1vvm;

[0056] 2) When adding sulfuric acid solution in step 1), when the oxidation...

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Abstract

The invention discloses a method for cultivating iron oxidized leaching microorganism. The method comprises the following steps: the leaching microorganism is cultured in a medium to the end of a logarithmic phase of the microorganism, the iron powder is added, the pH is adjusted, and the steps of stirring and aeration culture are stirred; adding calcium carbonate powder to adjust the pH to 2.0-2.5, stirring the material, and performing standing to obtain a supernatant and the sludge at a lower part, filtering the sludge to obtain filter residue and a filtrate, merging the supernatant and thefiltrate and performing continuous aeration culture; repeating the above steps, returning the filter residue to the next round of precipitation process until the concentration of microorganisms in thesupernatant reaches the requirement, and performing last culturing to obtain the supernatant and the filtrate obtained by filtering the sludge at the lower part as a bioleaching agent. The method employs a precipitation method to reduce the adsorption, agglomeration and retention of microorganisms on the solid surface, reduces the loss of microorganisms, and has good precipitation and filtrationproperty, and the concentration of microorganisms in the bioleaching agent reaches 109 cells / mL or more.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a method for cultivating iron oxide ore leaching microorganisms. Background technique [0002] With the continuous increase of society's demand for metal resources, mineral resources are becoming increasingly poor and miscellaneous. Realizing waste-free mining, protecting the environment, and recycling have become the objective requirements for sustainable development of resource development. At present, it is more and more difficult to develop and utilize traditional metallurgical technology, but biometallurgy technology has the characteristics of simple process flow, low cost, wide application range, safety and environmental protection, etc., and is expected to play an important role in green metallurgy and heavy metal waste harmless technology. Role. However, in industrial applications, the bioleaching cycle is usually long, and the problem of sl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C22B3/18C12R1/01
CPCC12N1/20C12N11/14C22B3/18Y02P10/20
Inventor 周洪波周文博程海娜刘荣辉彭晶陈辉葛杨田壮
Owner CENT SOUTH UNIV
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