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Preparation method for agaricus bisporus enzymatic hydrolysis peptide oral liquid

A technology of proteolysis and peptide oral liquid, which is applied in the field of nutrition and health products to achieve the effect of good taste, high nutritional value and good safety

Inactive Publication Date: 2018-12-11
罗铁柱
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the production of health products rich in active peptides with Agaricus bisporus has good commercial value and application prospects, but there are no reports about Agaricus bisporus proteolytic peptides and its products at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Raw material pretreatment: select fresh Agaricus bisporus raw materials, slice the raw materials of Agaricus bisporus into slices, mix them with water at a ratio of 1:1, beat for 2-3 minutes, and obtain Agaricus bisporus slurry.

[0020] Enzymolysis: put Agaricus bisporus slurry in an enzymolysis reactor, accurately weigh 0.58% acid protease and 0.22% papain, which account for the mass of Agaricus bisporus slurry, add them to the hydrolysis reactor, heat to 55°C, and adjust with 0.1M HCl solution Keep the pH value at 5.0, stir continuously during the enzymolysis process, and maintain a stable temperature and pH value, and enzymolysis for 5-6 hours.

[0021] Enzyme inactivation: When the protein is enzymatically hydrolyzed to a predetermined hydrolysis degree of 65%, stop stirring, remove the reactor, rapidly raise the temperature to 95°C, and heat for 10 minutes to inactivate the protease.

[0022] Centrifugal separation: the enzymatic hydrolysis solution was centrifuge...

Embodiment 2

[0027] Raw material pretreatment: Raw material pretreatment: select fresh Agaricus bisporus raw materials, slice the raw materials of Agaricus bisporus into slices, mix them with water at a ratio of 1:1, and beat to obtain Agaricus bisporus slurry.

[0028] Enzymolysis: put Agaricus bisporus slurry in an enzymolysis reactor, accurately weigh 0.55% acid protease and 0.20% papain, which account for the mass of Agaricus bisporus slurry, add them to the hydrolysis reactor, heat to 50°C, and adjust with 0.1M HCl solution Keep the pH value at 4.5, stir constantly during the enzymolysis process, and maintain a stable temperature and pH value, and enzymolysis for 5-6 hours.

[0029] Enzyme inactivation: When the protein is enzymatically hydrolyzed to 65% of the predetermined degree of hydrolysis, stop stirring, remove the reactor, rapidly raise the temperature to 95°C, and heat for 10 minutes to inactivate the protease;

[0030] Centrifugal separation: the enzymatic hydrolysis solutio...

Embodiment 3

[0035] Raw material pretreatment: Raw material pretreatment: select fresh Agaricus bisporus raw materials, slice the raw materials of Agaricus bisporus into slices, mix them with water at a ratio of 1:1.5, and beat to obtain Agaricus bisporus slurry.

[0036] Enzymolysis: put Agaricus bisporus slurry in an enzymolysis reactor, accurately weigh 0.6% acid protease and 0.25% papain, which account for the mass of Agaricus bisporus slurry, add them to the hydrolysis reactor, heat to 50°C, and adjust with 0.1M HCl solution Keep the pH value at 5.5, stir continuously during the enzymolysis process, and maintain a stable temperature and pH value, and enzymolysis for 5-6 hours.

[0037] Enzyme inactivation: When the protein is enzymatically hydrolyzed to 65% of the predetermined degree of hydrolysis, stop stirring, remove the reactor, rapidly raise the temperature to 95°C, and heat for 10 minutes to inactivate the protease;

[0038] Centrifugal separation: the enzymatic hydrolysis solu...

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PUM

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Abstract

The invention discloses a preparation method for an agaricus bisporus enzymatic hydrolysis peptide oral liquid. The method comprises the following steps: selecting and puling fresh agaricus bisporus raw materials so as to obtain an agaricus bisporus slurry, and putting the agaricus bisporus slurry into an enzymolysis reactor; accurately weighing acid proteinase and papayotin, adding the acid proteinase and the papayotin into a hydrolysis reactor, heating to 50-60 DEG C, regulating the pH value within a range of 4.5-5.5, and stirring for enzymolysis for 5-6 hours; while protein is hydrolyzed toa preset degree of hydrolysis of 65%, inactivating proteolytic enzyme, and performing centrifuging and concentration; and then performing blending, settling, encapsulation and sterilization, therebyobtaining a finished product. The agaricus bisporus enzymatic hydrolysis peptide oral liquid prepared by the method is high in peptide content, has gold color and good taste, and is free from fishy smell, good in product safety and high in nutrition value; the method has the advantages of simple process, easiness in operation, low equipment investment and low production cost, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of nutritional products and health products, and relates to an oral liquid using Agaricus bisporus as a raw material. Background technique [0002] Active peptides are currently a hot research topic in the international pharmaceutical and food circles and a functional factor with great development prospects. Lowering blood fat and other functions. At present, it has been used in biopharmaceuticals, health products, food, biological reagents, animal feed and other fields, such as insulin, cerebrolysin, collagen peptides, milk peptides, deer peptides, rice peptides, soybean peptides, egg white peptides, blood peptides, etc. [0003] Agaricus bisporus is an important edible fungus cultivated worldwide. China is the largest producer and exporter of Agaricus bisporus in the world, but its deep processing level is very backward, and there are almost no high value-added products. The protein content of Agaricus b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23J1/00A23J3/34A61K38/01A61K9/08A61P3/06A61P31/12A61P35/00A61P37/04
CPCA23J1/006A23J3/346A23V2002/00A61K9/0095A61K38/01A61K2236/19A61K2236/31A61K2236/51A61K2236/53A23L33/18A61P3/06A61P31/12A61P35/00A61P37/04A23V2200/30A23V2250/55
Inventor 罗铁柱
Owner 罗铁柱
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