CAPS marker related to wheat seed dormancy/preharvest sprouting tolerance and detection method thereof

Abstract

The invention discloses a CAPS marker related to wheat seed dormancy / preharvest sprouting tolerance and a detection method thereof. The CAPS marker is named as Qsd1-5BL, and the nucleotide sequence ofthe Qsd1-5BL is SEQ ID NO.1. The invention further provides a primer pair for amplifying the CAP maker and a method for identifying the duration of wheat seed dormancy / preharvest sprouting toleranceby utilizing the CAPS marker. After a wheat DNA is subjected to PCR amplification by utilizing the CAPS, digestion is carried out by utilizing HhaI restriction enzyme to obtain a digested product; when the digested product is a main belt, corresponding wheat seeds have a longer seed dormancy or longer sprouting tolerance duration; and when the digested product is two main belts, corresponding wheat seeds have shorter seed dormancy or shorter sprouting tolerance duration. The CAPS marker can provide gene resource and function mark for wheat varieties with stronger sprouting tolerance, and the tolerance hereditary basis can be widened.

Description

technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a gene related to wheat seed dormancy or ear germination resistance and a detection method thereof. Background technique [0002] Wheat is one of the most important food crops in the world, providing about 21% of food calories and 20% of protein for humans. China is the world's largest wheat producer and consumer. Therefore, high yield, stable yield and high quality have always been important goals of wheat breeding. However, if the wheat harvest period encounters rainy weather, it is easy to germinate on the ear (abbreviated as ear germination). If the rainy weather lasts for a long time, it will intensify the ear germination and seriously affect the yield and quality. Therefore, cultivating wheat varieties with long duration of resistance to panicle germination is particularly necessary, which is the fundamental way to reduce its damage; cloning genes with lon...

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Problems solved by technology

However, previous studies mostly focused on the effect of seed dormancy depth in a certain period of time after harvest on ear germination resistance, ignoring the fact that longer seed dormancy periods are resistant to ear germination persistence in the environment of longer duration of rainy wheat harvest season. the role of

Therefore, there are many reports on the depth (or level) of seed dormancy, such as the cloned Tasdr (2As / 2Bs, Zhang et al. 2014, 2017), TaVp-1 (3AL / 3BL, yang et al. 2007; Chang et al. 2011), TaMFT and TaPHS1 (3As, Nakamura et al. 2011; Liu et al. 2013) and PM19-A1 (4AL, Barrero et al. 2015) and TaMKK3-A (4AL, Torada et al. 2016); genes that regulate the duration of seed dormancy (referred to as dormancy) Fewer reports have hindered the cultivation of varieties with longer duration of panicle germination resistance to a certain extent

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