Acrylic acid grafting natural collagen with fibrillation performance and preparation method thereof

Abstract

The invention discloses acrylic acid grafting natural collagen with fibrillation performance and a preparation method thereof. The preparation method comprises the following steps of (1) dissolving natural collagen by acid solvents to obtain a natural collagen solution; (2) performing primary dialysis on the natural collagen solution obtained in the step (1) by using a phosphate buffer solution; removing acetic acid molecules; (3) dripping the phosphate buffer solution with dissolved N-hydroxysuccinimide acrylate or acrylic anhydride into the solution obtained in the step (2); performing uniform mixing for reaction; (4) performing secondary dialysis on the solution obtained after the reaction in the step (3); performing freeze drying to obtain the acrylic acid grafting natural collagen with fibrillation performance. Through simple and mild reaction conditions, the covalent bond grafting on the natural collagen can be realized; the grafting rate of the grafting natural collagen is regulated and controlled; the grafting modified natural collagen with fibrillation capability is obtained.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and more specifically relates to acrylic acid grafted natural collagen with fibrosis performance and a preparation method thereof. Background technique [0002] Collagen is a structural protein that exists widely in vertebrates. It has a triple helix structure formed by helical winding of three peptide chains. The molecular chain length is about 300 nm, the particle size is 1.5 nm, and the molecular weight is about 300,000 Da. Collagen is widely used in the field of biomaterials because of its excellent biological properties such as low immune activity, good biocompatibility, and biodegradability. However, the inherent mechanical properties, physical properties, gelation ability and biological stability of natural collagen still cannot fully meet the performance requirements of practical applications. At the same time, the rapidly developing fields of clinical medicine, tissue engineering an...

AI Technical Summary

Problems solved by technology

However, in the existing side chain graft modification process, the collagen triple helix structure is often destroyed due to harsh reaction conditions, such as high temperature, strong acid and alkali, or the collagen loses its ability to fibrillate due to too high a grafting rate, resulting in Unsatisfactory properties of grafted modified collagen

[0003] Collagen has broad application prospects in the field of biomedical materials, but as a protein, its mechanical properties are poor and its functional diversity is missing. Therefore, it is of great practical significance to optimize the grafting modification method to improve the mechanical properties and functional diversity of collagen.

However, there are still many deficiencies in the existing collagen modification technology, especially the graft modification operation often leads to the loss of collagen fibrosis ability

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