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Method for disinfecting Pseudomonas syringae pv. actinidiae in kiwifruit branches and nursery plant tissues

A technology of canker bacteria and disinfection method, which can be applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve problems such as inability to cure canker disease and plant resistance to drugs.

Active Publication Date: 2018-11-30
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological control is still in the screening of beneficial microorganisms and experimental stage of field control; the main agents commonly used in chemical control are copper hydroxide, kasugamycin and other pesticides. 1. The seedlings can only kill the Psa shown in the branches, and have no killing effect on the Psa in the branches and seedling tissues. The chemical pesticides currently used can only reduce the incidence of kiwifruit canker to a certain extent, but cannot cure the canker. There are no specific medicines available for trees and gardens with serious diseases, and the trees can only be dug up to destroy the garden; on the other hand, long-term and large-scale use of chemical pesticides will cause plants to develop resistance

Method used

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  • Method for disinfecting Pseudomonas syringae pv. actinidiae in kiwifruit branches and nursery plant tissues
  • Method for disinfecting Pseudomonas syringae pv. actinidiae in kiwifruit branches and nursery plant tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Choose kiwi fruit branches as the object to carry out the disinfection of canker bacteria. The specific method is:

[0018] (1) Cut 2-year-old or year-old branches of kiwi fruit into about 5cm;

[0019] (2) Put it into a watertight PE plastic bag, and then inject clear water into the PE plastic bag until the branches are submerged;

[0020] (3) Put the PE plastic bag with branches and clear water into the ultrasonic processor, when heated to a temperature of 45° C., ultrasonically treat for 45 minutes at an ultrasonic frequency of 80 Hz, and process the kiwifruit branch tissue;

[0021] (4) Immediately after the treatment, the branches were taken out and dried to complete the disinfection of the canker bacteria in the kiwifruit branch tissue, and the dried branches could be stored conventionally or directly used for grafting.

Embodiment 2

[0023] Choose kiwi fruit seedlings as the treatment object to carry out the disinfection of canker bacteria. The specific method is:

[0024] (1) 1-year-old or biennial kiwifruit seedlings are completely dug out from the kiwifruit nursery, and the aboveground part is cut off from the position of about 10cm above the rhizome junction;

[0025] (2) the nursery stock that cuts new shoot band root is put into watertight PE plastic bag, then injects clear water in PE plastic bag, until submerging whole nursery stock;

[0026] (3) Put the PE plastic bag that seedling stock and clear water are housed in ultrasonic processor, when heating to temperature 50 ℃, ultrasonic treatment 30min under the condition of ultrasonic frequency is 100Hz, kiwifruit branch tissue is processed;

[0027] (4) Immediately after the treatment, the seedlings are taken out of the PE belt to dry, and can be planted artificially or directly, and the seedlings can be sold.

Embodiment 3

[0029] Choose kiwi fruit seedlings as the treatment object to carry out the disinfection of canker bacteria. The specific method is:

[0030] (1) 1-year-old or biennial kiwifruit seedlings are completely dug out from the kiwifruit nursery, and the above-ground part is cut off new shoots from the position of 10 cm above the rhizome junction;

[0031] (2) the nursery stock that cuts new shoot band root is put into watertight PE plastic bag, then injects clear water in PE plastic bag, until submerging whole nursery stock;

[0032] (3) The PE plastic bag that seedling stock and clear water are housed is put into ultrasonic processor, when being heated to temperature 45 ℃, ultrasonic treatment is 50min under the condition that ultrasonic frequency is 70Hz, and kiwifruit branch tissue is processed;

[0033] (4) Immediately after the treatment, the seedlings are taken out of the PE belt to dry, and can be planted artificially or directly, and the seedlings can be sold.

[0034] The ...

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Abstract

The invention relates to a method for disinfecting Pseudomonas syringae pv. actinidiae in kiwifruit branches and nursery plant tissues, and the method mainly comprises putting the kiwifruit branches or nursery plants in a watertight PE plastic bag, injecting clear water into the PE plastic bag, placing the PE plastic bag in an ultrasonic sound field environment for ultrasonic treatment for 30 to 60 minutes at temperature of 45-50 DEG C under ultrasonic frequency of 60 to 100 Hz, taking the branches or the nursery plants out to dry in air to complete the disinfecting of the Pseudomonas syringaepv. actinidiae in the kiwifruit branches and the nursery plant tissues. The method aims at the Pseudomonas syringae pv. actinidiae Psa in the branch tissues such as cortex, phloem and xylem vessels of the branches, scion spreading of the Pseudomonas syringae pv. actinidiae Psa of the kiwifruit nursery plants can be effectively prevented, the sterilizing rate for the Pseudomonas syringae pv. actinidiae Psa in the biennial or annual branch tissues of the method can reach 97.75%, but the method does no effect normal growth of the nursery plants.

Description

technical field [0001] The invention relates to heat+ultrasonic treatment for disinfecting canker bacteria in kiwifruit branches and seedling tissues, which belongs to the comprehensive prevention and control technology of kiwifruit canker, and is especially suitable for the comprehensive prevention and control of canker in new development areas of kiwifruit. Background technique [0002] Kiwifruit bacterial canker (kiwifruit bacterial canker) is a kind of disease that can cause its industry to be destructive. The world's kiwifruit-producing areas of New Zealand, Spain, Italy, Turkey and China's kiwifruit-producing areas are located in 16 provinces of Shaanxi, Sichuan, and Anhui. Canker damage has occurred in all planting areas, which seriously threatens the development of the world and China's kiwifruit industry. In China, New Zealand, the United States and the European Plant Protection Organization (EPPO), kiwifruit canker has been regarded as a plant quarantine object and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 高贵田封琦张鑫刘芸宏孙强
Owner SHAANXI NORMAL UNIV
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