Test sample allergenicity detection method and kit thereof

Abstract

The invention relates to a test sample allergenicity detection method and a kit thereof. The detection method comprises the following steps: randomly dividing a plurality of mice into groups; measuring the thickness of ears of each mouse of different groups; entering into a first time period; within the first time period, smear an equal amount of a reagent on the ears of each mouse of different groups; entering into a second time period; when the second time period is ended, firstly measuring the thicknesses of the ears of the mice of different groups, further killing the mice, taking down lymph glands after the ears of the mice under a sterile condition, preparing a cell suspension, putting a 5-bromodeoxyuridine solution into the cell suspension, culturing, and testing the content of the5-bromodeoxyuridine by using an ELISA (Enzyme-Linked Immuno Sorbent Assay) method; and judging the allergenicity of a test substance according to limiting standards. By adopting the method, whether the test substance has positive allergenicity can be tested, intraperitoneal injection is avoided, then animals can be prevented from pain, the amount of the 5-bromodeoxyuridine can be greatly reduced,and the detection cost can be reduced.

Description

technical field [0001] The invention relates to a method for detecting the allergy of a test substance and a kit thereof, belonging to the technical field of medical detection. Background technique [0002] As far as the inventors know, when sensitizing exogenous chemicals stimulate the body, the body is prone to produce an allergic reaction, and to determine whether a chemical is sensitizing, it needs to be detected by animal experiments. [0003] The currently commonly used detection method uses the method of intraperitoneally injecting the test substance into the animal, but this will cause pain to the animal and does not conform to the "3R" principle of animal welfare. At the same time, the more expensive 5-bromodeoxyuridine (BrdU) will be used in this detection method, and the dosage of each mouse can reach 5 mg, and a detection test uses a minimum of 20 mice per group, that is, every The group requires at least 100mg of BrdU, making the entire assay cost prohibitively...

AI Technical Summary

Problems solved by technology

[0004] After searching, it was found that in the prior art represented by the Chinese invention patent application with the application number CN201711285287.7, the application publication number CN107966562A, and the name "A Method for Detection and Evaluation of Allergenicity of Recombinant Coxsackievirus B3 Injection", and No technical means or technical teachings to solve the above technical problems

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