A method for judging and detecting the similarity of Dendrobium candidum pure species

Abstract

The invention relates to a method for judging and detecting the similarity of pure Dendrobium officinale. The establishment process of the judging method is as follows: S1: collect samples of Dendrobium officinale that are consistent with the conclusion of gene sequencing, and measure the stem diameter of each sample; S2: pair Sample stem diameter variable carries out normality test; S3: standard interval: if the result obtained in step S2 obeys normal distribution, then obtain the 95% confidence interval of mean value and 95% of standard deviation according to the general calculation formula of normal distribution. % confidence interval; this confidence interval can be used as a standard range to identify the purity of unknown samples. The judgment standard established according to the actual collected data is: the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are respectively: (5.8079, 6.3227) and (0.9256, 1.2954), the tested Dendrobium officinale sample only needs to be in accordance with this interval Just compare. The invention can identify the purity of the Dendrobium officinale through its morphology-related characteristics, thereby simply judging the value or existence of the artificially planted Dendrobium officinale; the identification has high accuracy and has important practical significance.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to a method for judging and detecting the similarity of pure Dendrobium officinale, that is, a method for establishing the correlation between the morphological characteristics of Dendrobium candidum and the conclusion of gene sequencing and the method for determining the purity of the tested species of Dendrobium officinale identification method, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium officinale, Dendrobium fringe, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was listed as a top-grade nourishing product in "Shen Nong's...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium candidum.

However, long-term artificial planting has also brought false ones to false ones and shoddy good ones. This is because ① artificial planting has changed the growth environment of wild Dendrobium candidum; The spread of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium candidum; ③And because there are many varieties of Dendrobium, the hybridization between the varieties makes the phenomenon of trait crossover among its close relatives; ④Others are uncontrollable or unmeasurable In summary, the medicinal ingredients of some artificially planted Dendrobium officinale have changed, and even important medicinal ingredients have disappeared. There are substantial differences in the wild gene sequence

Once the medicinal value of these Dendrobium candidum weakens or disappears, and the field continues to use it without knowing it, the consequences will be very serious. more serious consequences

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