A method for judging and detecting the similarity of pure Dendrobium nobile

Abstract

The invention relates to a method for judging and detecting the similarity of pure Dendrobium nobile. The establishment process of the judging method is as follows: S1: collect samples of Dendrobium nobile that are consistent with the conclusion of gene sequencing, and measure the second internode of each sample. Stem diameter; S2: Carry out normality test on the stem diameter variable in the second internode of the sample; S3: Standard interval: If the result obtained in step S2 obeys the normal distribution, then the mean value is obtained according to the overall calculation formula of the normal distribution The 95% confidence interval and the 95% confidence interval of the standard deviation; this confidence interval can be used as a standard range to identify the purity of unknown samples. The judgment standard established according to the data actually collected is: the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are respectively: (8.3677, 9.3422) and (2.4923, 3.1872), the tested Dendrobium nobile sample only needs to be compared with the Compare intervals. The present invention can identify the purity of the species of Dendrobium nobile through its morphology-related characteristics, so that it can simply judge the value or existence of the artificially planted Dendrobium nobile; the identification accuracy is high, and it has important practical significance .

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to a method for judging and detecting the similarity of pure Dendrobium nobile, that is, a method for establishing the correlation between the morphological characteristics of Dendrobium nobile and the conclusion of gene sequencing and the purity of the tested Dendrobium nobile species. degree of identification methods, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. The genus Dendrobium is the largest genus in the Orchidaceae plant, including Dendrobium glabra, Dendrobium fringe, Dendrobium officinale, Dendrobium multiflora, Dendrobium dendrobii, and Dendrobium nobile. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was listed as a top-grade nourishing product in "Shen Nong's Mat...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium nobile

However, the long-term artificial planting has also brought about false ones and substandard ones for Dendrobium nobile. This is because ① artificial planting has changed the growth environment of wild Dendrobium nobile; , the application of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium nobile; ③ and because there are many varieties of Dendrobium, the hybridization between its varieties makes the traits of its close relatives cross; ④ other uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium nobile have changed, and even important medicinal ingredients have disappeared. The gene sequence is also substantially different from the original wild gene sequence

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