Establishment of pure Dendrobium huoshanense variety similarity judging method, pure Dendrobium huoshanense variety similarity judging standard and pure Dendrobium huoshanense variety similarity judging method

Abstract

The invention relates to an establishment of a pure Dendrobium huoshanense variety similarity judging method, a pure Dendrobium huoshanense variety similarity judging standard and a pure Dendrobium huoshanense variety similarity judging method. The establishment process of the method comprises the following steps: S1, collecting samples of Dendrobium huoshanense consistent with the gene sequencingconclusion, and measuring the second internode length of the stem of each sample; S2, performing normality test on the second internode length of the stem of each sample; and S3, standard interval: if the results obtained in the step S2 obey the normal distribution, obtaining 95% confidence interval of a mean and 95% confidence interval of a standard deviation according to the normal distributionpopulation calculation formula, wherein the confidence intervals can be used as a standard range for identifying the purity of unknown samples. The judging standard established according to the collected data is as follows: 95% confidence interval of a mean and 95% confidence interval of a standard deviation are (9.8124, 11.2212) and (3.4092, 4.4147), respectively, and the tested Dendrobium huoshanense samples only need to be compared with the intervals. The invention can identify the purity of the variety through the related morphological characteristics of Dendrobium huoshanense, so that the value or the existence of artificially planted Dendrobium huoshanense can be simply judged. The accuracy of identification is high, which has important practical significance.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to the establishment of a method for judging the similarity of Dendrobium huoshanense purebred, the judgment standard and the judgment method, that is, the method for establishing the correlation between the morphological characteristics of Dendrobium huoshanense and the conclusion of gene sequencing and the method to be tested The identification method of the purity of Dendrobium huoshanense, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium Huoshanense, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicin...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium Huoshanense.

However, long-term artificial planting has also brought fakes to Huoshanense Dendrobium, because ① artificial planting has changed the growth environment of wild Dendrobium Huoshanense; The spread of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium huoshanense; ③and because there are many varieties of dendrobium, the hybridization between the varieties makes the phenomenon of trait crossover among its close relatives; ④Others are uncontrollable or unmeasurable In summary, the medicinal ingredients of some artificially planted Dendrobium Huoshanense have changed, and even important medicinal ingredients have disappeared. There are substantial differences in the wild gene sequence

Once the medicinal value of Dendrobium Huoshanense weakens or disappears, and the field continues to use it without knowing it, the consequences will be very serious. more serious consequences

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