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Method for detecting five sweetening agents in tobacco essence through ultra-high performance liquid chromatography-tandem mass spectrometry

An ultra-high performance liquid phase and tandem mass spectrometry technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve complex problems, achieve the effects of reducing interference, simple operation, and simple pretreatment methods

Active Publication Date: 2018-11-23
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the matrix of flavors is more complex than that of beverages and dairy products, many of them are natural extracts, which cannot be accurately qualitative and quantitative by existing methods

Method used

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  • Method for detecting five sweetening agents in tobacco essence through ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for detecting five sweetening agents in tobacco essence through ultra-high performance liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] 1. Instruments and reagents:

[0037] The 5 sweeteners and methanol are all chromatographic grade reagents; they meet the requirements of first-grade water in GB / T 6682.

[0038] Waters TQD quadrupole tandem mass spectrometer; Swiss Mettler AE 163 electronic balance (sensitivity: 0.0001g).

[0039] 2. Sample handling:

[0040] Weigh 0.2 g of tobacco flavor sample (accurate to 0.01 g) and place it in a 50 mL Erlenmeyer flask with a stopper. Accurately add 10 mL of extract: ultrapure water, shake and extract for 10 min; sample purification: stand still for 5 min, draw 2 mL of supernatant into a 5 mL centrifuge tube, and add 25 mg of C18 powder to the centrifuge tube, vortex After 2 min, the supernatant was filtered through a 0.22 μm aqueous membrane filter. Pipette 100 μL of the filtrate, dilute the sample to 1.0 mL with ultrapure water, and use it as the sample solution for UPLC-MS / MS analysis;

[0041] 3. Prepare the standard working solution: see the content of t...

example 2

[0046] As the method described in Example 1, another tobacco flavor sample was selected, and the above five sweeteners were not detected.

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Abstract

The invention belongs to the technical field of physical and chemical inspection of food additive residues in tobacco essences, and particularly relates to a method for detecting five sweetening agents in a tobacco essence through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps that the tobacco essence is weighed, then ultrapure water is added, then oscillating extraction is carried out, extract liquid is purified and filtered and is diluted by using the ultra-pure water, and then the sweetening agents inthe tobacco essence are directly detected through the ultra-high performance liquid chromatography-tandem mass spectrometry. The method has the advantages of being capable of rapidly and accurately detecting the residual quantity of the sweetening agents in the tobacco essence, environmentally friendly, accurate in result, less in interference, high in sensitivity and good in repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical inspection of food additive residues in tobacco essence, and mainly relates to the determination method of five kinds of sweeteners in tobacco essence. Direct method of mass spectrometry. Background technique [0002] Sweeteners are an important class of food additives used to improve the taste and flavor of food. Among them, non-sugar sweeteners have become substitutes for sucrose due to their advantages of high sweetness, low calorie, and not participating in metabolic processes. Widely used in industry. In recent years, with the improvement of consumption and awareness, people pay more and more attention to food safety issues, and the use of various additives in food, especially sweeteners, has attracted more and more attention. Food additives are harmless to humans when used within the specified dosage range, but if used in excess, it may cause various forms of toxicity. There...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨飞王颖刘珊珊范子彦李中皓邓惠敏边照阳唐纲岭
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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