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Human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line and establishing method and application thereof

An epidermal growth factor and tyrosine kinase technology, which is applied in the field of human epidermal growth factor tyrosine kinase inhibitor-acquired drug-resistant lung cancer cell lines and its establishment, can solve the problem that drug-resistant cell lines cannot respond to drug resistance mechanisms, resistance to The stability of the biological properties of the drug cell lines is not good, and the tumor tissue is not easy to obtain, so as to achieve the effects of easy acquisition, stable biological properties and strong vitality

Active Publication Date: 2018-11-23
SHANGHAI PULMONARY HOSPITAL
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Problems solved by technology

[0006] The present invention is mainly aimed at the technical problem that the tumor tissue is not easy to obtain in the primary culture of lung cancer cell lines, the problem that the drug-resistant cell line induced by the current method cannot reflect the real drug resistance mechanism, and the biological characteristics of the drug-resistant cell line The problem of poor stability provides a new human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistant lung cancer cell line and its establishment method and application

Method used

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  • Human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line and establishing method and application thereof
  • Human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line and establishing method and application thereof
  • Human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line and establishing method and application thereof

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Embodiment 1

[0035] 1 Experimental materials

[0036]DMEM medium: purchased from HyClone Company;

[0037] Fetal bovine serum (FBS) is a product of GIBCO;

[0038] BALB / c-nu mice: purchased from Shanghai Slack Experimental Animal Co., Ltd.;

[0039] Gefitinib API: purchased from Dalian Meilun Biotechnology Co., Ltd.;

[0040] Matrigel: purchased from Corning;

[0041] Other sources of reagents are:

[0042]

[0043] 2 Experimental methods

[0044] Among the inpatients in Shanghai Pulmonary Hospital, looking for lung cancer patients with acquired drug resistance after one year of oral gefitinib treatment, extract 400-500ml of pleural effusion (non-small cell lung cancer cells found in pleural effusion: adenocarcinoma), room temperature Settling for 1 hour. The precipitate was collected, centrifuged at 1500 rpm for 10 min, and the supernatant was carefully removed. Add PBS, centrifuge at 1500rpm for 10min and wash three times. Take a small amount of precipitate and smear it on a g...

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Abstract

The invention relates to a human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line and an establishing method and application thereof. The establishing method comprises the following steps: collecting and confirming precipitates containing cancer cells from pleural fluid of a lung cancer patient with human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance, culturing in vitro, inoculating to mouse underarm, taking tumor tissue after tumor formation, carrying out primary culture, and confirming drug-resistance tumor cells with purity being more than 90%, thereby establishing the human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance lung cancer cell line with preservation number of CCTCC NO: C2018121. The cell line keeps main clinical biological characteristics, can reflect a drug resistance mechanism more truly, has a high tumor formation rate, stable biological characteristics and strong vitality, and is an ideal cell line material for basic research and preclinical testing of human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistance.

Description

technical field [0001] The invention relates to the technical field of drug-resistant cell lines, in particular to a human epidermal growth factor tyrosine kinase inhibitor acquired drug-resistant lung cancer cell line and its establishment method and application. Background technique [0002] In recent years, with the development of modern industry, the deterioration of people's living environment, the impact of bad living habits and the aging population, the incidence of lung cancer has increased year by year. In 2016, CA Cancer J Clin published the 2015 cancer statistics in China. In 2015, there were about 733,300 new lung cancer cases in my country, ranking first among the top ten tumors in terms of incidence and mortality. Although the level of diagnosis and treatment of lung cancer has been greatly improved compared with the past, lung cancer is still one of the malignant tumors with the highest mortality rate, and the 5-year survival rate of lung cancer is only 16.1%....

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02
CPCC12N5/0693C12N2509/00G01N33/5017G01N33/5044
Inventor 周崧雯邓沁芳谢博雄季现秀方启宇孙辉鲍昱辰
Owner SHANGHAI PULMONARY HOSPITAL
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