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Colonic adenocarcinoma susceptibility prediction kit and system

A colon adenocarcinoma, susceptibility technology, applied in the field of biomedicine, can solve problems such as unscientific, impossible, and inability to early warning of tumor susceptibility

Active Publication Date: 2018-11-20
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the occurrence and development of tumors is a very complicated process, and it is obviously impossible and unscientific to use the changes of a single molecular genetic marker to diagnose the disease
With the existing technical means, it is not possible to provide a more accurate early warning of tumor susceptibility only through genetic information, and the current early identification and prediction methods for tumors need to be improved

Method used

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  • Colonic adenocarcinoma susceptibility prediction kit and system
  • Colonic adenocarcinoma susceptibility prediction kit and system
  • Colonic adenocarcinoma susceptibility prediction kit and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Example 1 A Colon Adenocarcinoma Susceptibility Prediction System

[0111] A colon adenocarcinoma susceptibility prediction system comprising:

[0112] A device for obtaining the number of repetitions of the STR site short tandem sequence of the sample DNA;

[0113] Data processing and judging device, it comprises following module (as figure 1 ):

[0114] The data input module is used to input the age, gender, and repeat times of the short tandem sequence of the subject;

[0115] The database management module is used for the operation management of data storage, modification, deletion, query and printing;

[0116] The data calculation module is used to calculate the result of the discriminant function according to the number of repetitions of the STR site short tandem sequence in the data input module;

[0117] The analysis, discrimination and result output module is used to compare the results of the discriminant function, so as to predict the susceptibility of c...

Embodiment 2

[0152] Example 2 A colon adenocarcinoma susceptibility prediction kit

[0153] A colon adenocarcinoma susceptibility prediction kit comprising the following components: STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, STR-6 primers, PCR amplification Reaction solution, LIZ-500 molecular weight internal standard, deionized formamide, instruction manual.

[0154] The concentrations of the above STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers are all 10 μM, and the primer sequences are shown in the following table:

[0155]

[0156]

[0157] The PCR amplification reaction solution is a mixture of the following reagents: TaqDNA polymerase (5U / μL), Tris-HCl (100mM, pH 8.8 at 25°C), KCl (500mM), ethylphenyl polyethylene glycol (0.8 %(v / v)), MgCl 2 (25mM), dNTP (10mM), deionized water.

[0158] The PCR amplification reaction solution is stored at -20°C; the LIZ-500 molecular weight internal standard is st...

Embodiment 3

[0160] Example 3 Using the system of Example 1 and the kit of Example 2 to predict the risk of the subject suffering from colon adenocarcinoma

[0161] Subject: Male, 74 years old, was treated at the Department of Colorectal and Anal Surgery of the Second Hospital of Jilin University. After being fully informed of the purpose and purpose of the examination and voluntary, he signed an informed consent and collected 1 mL of anticoagulant blood via a peripheral vein. .

[0162] Use the kit of embodiment 2, carry out the following steps according to the method recorded on the kit instruction manual:

[0163] (1) Extract sample DNA: Use a DNA extraction kit to extract blood genomic DNA;

[0164] (2) PCR reaction

[0165] (2-1) Take out STR-1 primer, STR-2 primer, STR-3 primer, STR-4 primer, STR-5 primer, STR-6 primer, PCR amplification reaction solution from the refrigerator, equilibrate to room temperature, each After the components are fully dissolved, quickly centrifuge for 1...

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Abstract

The invention relates to a colonic adenocarcinoma susceptibility prediction kit which comprises the following components: an STR-1 primer, an STR-2 primer, an STR-3 primer, an STR-4 primer, an STR-5 primer and an STR-6 primer, and further can comprise a PCR amplification reaction solution, an LIZ-500 molecular weight internal standard and deionized formamide. The colonic adenocarcinoma susceptibility prediction kit provided by the invention can be used for diagnosing colonic adenocarcinoma and predicting susceptibility. The invention also provides a colonic adenocarcinoma susceptibility prediction system.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, it relates to a colon adenocarcinoma susceptibility prediction kit and a colon adenocarcinoma susceptibility prediction system. More specifically, the present invention relates to a kit for detecting STRs of colon adenocarcinoma susceptibility-related genes by means of short tandem repeats (Short tandem repeats, referred to as STR) site fragment analysis methods, and by combining discriminant analysis statistical methods, thereby Early warning of susceptibility to colon adenocarcinoma in subjects. Background technique [0002] Tumor is a disease closely related to genetics. To study the molecular genetic basis of tumors, and then propose tumor-specific genetic markers, it is hoped that it can be used for routine detection, clinical diagnosis, personalized treatment, and disease tracking after recovery. Etc. provides a simple and feasible method. However, the variability of indi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 郝书弘王晓峰任明杨麒巍
Owner JILIN UNIV
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