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Method for evaluating nitrate ion absorption kinetics of Acorus gramineus

A technology of nitrate ion and calamus, applied in the field of evaluating the kinetics of nitrate ion absorption by calamus, can solve the problems of one-sidedness and improper application, and achieve the effect of expanding the scope of application and changing the one-sided evaluation of kinetic characteristics

Inactive Publication Date: 2018-11-09
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the accompanying ions are not considered, the application of plants according to the kinetic parameters will be relatively one-sided, resulting in improper application

Method used

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  • Method for evaluating nitrate ion absorption kinetics of Acorus gramineus
  • Method for evaluating nitrate ion absorption kinetics of Acorus gramineus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Select a number of calamus with the same plant height, good growth conditions, and no pests and diseases, and put them in 1 / 2 Hoagland nutrient solution (pH 5.8) for 1 month.

[0041] 2) Take out the calamus cultivated in step 1), and rinse the root with deionized water.

[0042] 3) the calamus calamus of step 2) is processed, changes into the CaSO of 0.2mmol / L 4solution, pre-incubated for 48h to achieve starvation.

[0043] 4) Transfer the starved calamus treated in step 3) into a black plastic bucket containing 1.5 L of absorption liquid. The composition of the absorption liquid is 1mmol / L NaNO 3 +0.2mmol / L CaSO 4 , and each absorption experiment was repeated 3 times.

[0044] 5) At 0, 1, 2, 3, 5, 7, and 9 hours, draw 10ml of absorption liquid from the plastic bucket, add 10ml of distilled water after sampling.

[0045] 6) Measure NO in the absorption liquid taken out in step 5) 3 - content.

[0046] 7) After the absorption experiment, the roots were cut of...

Embodiment 2

[0050] 1) Select Acorus calamus with consistent plant height, good growth conditions, and no pests and diseases, and put them in 1 / 2 Hoagland nutrient solution (pH5.8) for one month.

[0051] 2) Take out the calamus cultivated in step 1), and rinse the root with deionized water.

[0052] 3) the calamus calamus of step 2) is processed, changes into the CaSO of 0.2mmol / L 4 solution, pre-incubated for 48h to achieve starvation.

[0053] 4) Transfer the starved calamus treated in step 3) into a black plastic bucket containing 1.5 L of absorption liquid. The composition of the absorption liquid is 1mmol / L NH 4 NO 3 +0.2mmol / L CaSO 4 , and each absorption experiment was repeated 3 times.

[0054] 5) At 0, 1, 2, 3, 5, 7, and 9 hours, draw 10ml of absorption liquid from the plastic bucket, add 10ml of distilled water after sampling.

[0055] 6) Measure NO in the absorption liquid taken out in step 5) 3 - content.

[0056] 7) After the absorption experiment, the roots were cut...

Embodiment 3

[0060] 1) Select Acorus calamus with consistent plant height, good growth conditions, and no pests and diseases, and put them in 1 / 2 Hoagland nutrient solution (pH5.8) for one month.

[0061] 2) Take out the calamus cultivated in step 1), and rinse the root with deionized water.

[0062] 3) the calamus calamus of step 2) is processed, changes into the CaSO of 0.2mmol / L 4 solution, pre-incubated for 48h to achieve starvation.

[0063] 4) Transfer the starved calamus treated in step 3) into a black plastic bucket containing 1.5 L of absorption liquid. The composition of the absorption liquid is 1mmol / L KNO 3 +0.2mmol / L CaSO 4 , and each absorption experiment was repeated 3 times.

[0064] 5) At 0, 1, 2, 3, 5, 7, and 9 hours, draw 10ml of absorption liquid from the plastic bucket, add 10ml of distilled water after sampling.

[0065] 6) Measure NO in the absorption liquid taken out in step 5) 3 - content.

[0066] 7) After the absorption experiment, the roots were cut off,...

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Abstract

The invention discloses a method for evaluating the nitrate ion absorption kinetics of Acorus gramineus. An ion consumption curve equation about the absorption of NO<3><-> by the Acorus gramineus in the presence of different adjoint ions, and the absorption dynamics parameters Imax and Km of the Acorus gramineus in the presence of different adjoint ions are calculated. The absorption dynamics parameters of the Acorus gramineus in the presence of different adjoint ions are studied, so a plant can be correctly selected according to the ion components of water in order to enlarge the adaption range of in the presence of different adjoint ions nutrients. The method also can be used to guide the scientific application of the Acorus gramineus in eutrophic water containing different components inorder to make the Acorus gramineus play a great role in every stage of removal of NO<3><-> form the eutrophic water. The components of the adjoint ions can be changed artificially to enhance the pollutant absorption ability of the plant.

Description

technical field [0001] The invention belongs to the field of plant water restoration, and more specifically relates to a method for evaluating kinetics of nitrate ion absorption by calamus calamus. Background technique [0002] Water pollution caused by excessive nitrogen, phosphorus and other nutrients in the water body is the phenomenon of water body eutrophication. Water eutrophication has become a global water environment problem. In recent years, the use of plant ecosystems to purify nutrient water bodies has become a key technology for constructing and restoring aquatic ecosystems. Aquatic plants can absorb and utilize ammonium nitrogen and nitrate nitrogen in eutrophic water for their own physiological metabolism, reduce the nitrogen content in eutrophic water, and purify the water. Phytoremediation is a low-cost, easy-to-operate, and effective phytoremediation method to remove excessive nutrients in water. [0003] Iris (Acorus tatarinowii Scho) is araceae, Acorus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00G01N33/18
CPCG01N30/00G01N33/182
Inventor 于金平李俊林周冬琴贾明云
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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