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A method of spraying microbial preparations for preventing and controlling plant diseases

A microbial preparation and plant disease technology, applied in the direction of microorganism-based methods, plant growth regulators, plant growth regulators, etc., can solve environmental hazards such as weakened drug resistance, pathogenic bacteria resistance, soil, water flow, and air and other problems, to achieve good control effect and improve the effect of strain viability

Active Publication Date: 2019-05-17
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and treatment of Botrytis cinerea mainly relies on chemical means, but the long-term use of chemical pesticides not only easily leads to the development of drug resistance of pathogenic bacteria and weakens the disease resistance of pesticides, but also causes serious harm to the environment such as soil, water flow, air, etc. A range of social and ecological issues

Method used

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  • A method of spraying microbial preparations for preventing and controlling plant diseases
  • A method of spraying microbial preparations for preventing and controlling plant diseases
  • A method of spraying microbial preparations for preventing and controlling plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Wuyiencin and bacillus plate confrontation test (plate antagonism test)

[0037] In this part, the zone of inhibition method was used to determine the inhibitory effect of different concentrations of Wuyiencin on Bacillus subtilis, Bacillus polymyxa and methylotrophic Bacillus.

[0038] (1) Test strains

[0039] Methylotrophic Bacillus (Bacillus methylotrophicus): from the Agricultural Antibiotics Research Group of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, isolated from the Changbai Mountain area of ​​Jilin Province; Bacillus subtilis: isolated from the unit cell of the product provided by Byward Co., Ltd. of the United States and 16s identification; Bacillus polymyxa (Paenibacillus polymyxa): obtained by the product unit cell isolation and 16s identification provided by Lulong Biotechnology Co., Ltd.

[0040] (2) Preparation and quantification of Wuyiencin and Bacillus suspension

[0041] Preparation of Wuyiencin fermenta...

Embodiment 2

[0050] Embodiment 2: Wuyiencin Determination of Minimum Inhibitory Concentration of Bacillus

[0051] In Example 1, it was found that Wuyiencin had no antagonistic effect on Bacillus at 500 ppm, and antagonism occurred when the concentration rose to 1000 ppm. In order to clarify that Wuyiencin is the minimum inhibitory concentration for Bacillus, this study diluted Wuyiencin fermentation broth to 600ppm, 700ppm, 800ppm, 900ppm and 1000ppm for use. The minimum inhibitory concentration test of Wuyiencin was carried out by using the above-mentioned plate confrontation test method.

[0052] Take 100 μL of methylotrophic Bacillus, Bacillus subtilis and Bacillus polymyxa suspension respectively in 3 bottles of LB liquid medium, shake well and pour plate, pour 20mL medium into each plate. Draw a cross on the back of the petri dish. After the culture medium is solidified, use tweezers to gently place a sterile Oxford cup into the intersection of the cross on the petri dish. Pipette ...

Embodiment 3

[0056] Example 3: Combined application of Wuyiencin and methylotrophic bacillus to control cucumber powdery mildew in protected ground

[0057] Both Examples 1 and 2 show that Wuyiencin has better compatibility with methylotrophic Bacillus. In this study, Wuyienin and methylotrophic Bacillus were applied in combination to study its control effect on powdery mildew. The difference between the two alone.

[0058] (1) Drug preparation

[0059] Preparation of Wuyiencin: inoculate Wuyiencin-producing bacteria in YEME medium for 2 days at 28°C on a constant temperature shaker, then inoculate in the fermentation medium, continue to culture at 28°C on a constant temperature shaker for 64 hours, and then filter the fermentation broth to obtain the supernatant That is Wuyiencin fermentation liquid. The specific formula of the culture medium is the same as in Example 1. Pass the fermented wuyiencin fermented liquid through a 0.22 μm microporous filter to obtain a sterile fermented liq...

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Abstract

The invention provides a method of spraying a microbial preparation for preventing and controlling plant diseases. The method comprises the following steps of step 1, performing root-irrigation treatment on crops to be prevented and treated with a bacteria solution of methylotrophy bacillus; step 2, performing foliage spraying on the crops to be prevented and treated with a bacteria solution of wuyiencin; and step 3, repeating steps from the step 1 to the step 2 for totally 3-4 times. The method has the beneficial effects that the two of the wuyiencin and the bacillus have good prevention andcontrol effects to the vegetable fungal diseases, have own considerations on the effect of preventing and controlling the diseases and perform implementation operation through combination by adoptingmanners of root irrigation with the methylotrophy bacillus and foliage spraying with the wuyiencin, so that the prevention and control effects to social crop diseases of the two of the wuyiencin and the bacillus are exerted to a greater extent, and a more effective matching application manner is provided to production of vegetable crops of protected areas.

Description

technical field [0001] The invention relates to the technical field of application of microbial agents, in particular to a method for spraying microbial agents for preventing and controlling plant diseases. Background technique [0002] As an important vegetable crop in my country, cucumber is one of the important pillar industries in modern agricultural vegetable production, and its cultivation area is increasing continuously. In recent years, with the promotion of the greenhouse planting model in protected areas, the occurrence of cucumber powdery mildew has become increasingly serious due to special climatic conditions such as high temperature and high humidity in the greenhouse. Every year, the crop yield due to cucumber powdery mildew is reduced by about 10%, and the production capacity reduction in epidemic years reaches about 20%. Cucumber powdery mildew is a fungal disease caused by Ascomycota monofilament powdery mildew. In the early stage of the disease, dot-shape...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N43/54A01P21/00A01P3/00C12R1/07
CPCA01N43/54A01N63/00C12N1/20
Inventor 张克诚葛蓓孛刘炳花麻金金施李鸣赵文珺姜明国
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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