Ag+ fluorescent probe and Ag+ chemical sensor

A fluorescent probe and sensor technology, applied in the field of fluorescent probes, to achieve the effects of colorimetric detection with naked eyes, reversible and non-destructive fluorescence, and bright colors

Inactive Publication Date: 2018-11-06
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of Ag + Fluorescent probe and preparation method thereof, aiming at solving existing Ag + Fluorescent probes cannot simultaneously take into account high selectivity, high sensitivity, and environmental protection and recycling

Method used

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  • Ag+ fluorescent probe and Ag+ chemical sensor
  • Ag+ fluorescent probe and Ag+ chemical sensor
  • Ag+ fluorescent probe and Ag+ chemical sensor

Examples

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preparation example Construction

[0042] Correspondingly, another aspect of the embodiment of the present invention provides an Ag + The preparation method of fluorescent probe comprises the following steps:

[0043] S01. Under the protection of nitrogen or inert gas, add compound 1 shown in the following structure, anhydrous acetone, and cesium carbonate to a dry reaction bottle, and perform the first heating and reflux treatment; after cooling, add compound 2 and iodide shown in the following structure Cesium is heated and refluxed for the second time to prepare compound 3 shown in the following structure, and the reaction formula is as follows:

[0044]

[0045] S02. Under the protection of nitrogen or inert gas, add compound 3, absolute ethanol, and hydrazine hydrate to a dry reaction bottle, heat and reflux for the third time, filter out the white insoluble solid after the reaction, and evaporate the filtrate to dryness with chloroform methanol Recrystallize to prepare compound 4 shown in the followin...

Embodiment 1

[0071] a kind of Ag + The preparation method of fluorescent probe (hereinafter referred to as probe), comprises the following steps:

[0072] S11. Synthesis of compound 3

[0073] Under nitrogen protection, compound 1 (500mg, 0.48mmol) and 100mL of dry acetone were added to a dry 250ml three-neck flask, stirred to dissolve, cesium carbonate (1.56g, 4.8mmol) was added, and heated to reflux for 1h. After it was cooled, compound 2 (264mg, 1.2mmol) and cesium iodide (124mg, 0.48mmol) were added, and heated to reflux for 6h. After the reaction was completed, the solvent was evaporated to dryness, extracted with chloroform (3×100 mL), the organic phase was washed with 200 mL of saturated brine, dried over anhydrous magnesium sulfate, and the solvent was evaporated under reduced pressure. Chloroform / methanol (100 / :2) was separated and purified by silica gel column chromatography to obtain 0.46 g of yellow solid compound 3 with a yield of 67.3%.

[0074] The NMR data of compound 3 ...

Embodiment 2

[0088] The preparation method of various reagents in the analytical method of the present invention:

[0089] (1) Preparation of probe stock solution: Weigh 75.1 mg of the probe reagent prepared in Example 1, dissolve it with N,N-dimethylformamide and acetonitrile respectively, and prepare Ag +Fluorescent probe concentration of 1mM N,N-dimethylformamide solution (N,N-dimethylformamide probe stock solution) 50mL, Ag + Fluorescent probe concentration of 1mM acetonitrile solution (acetonitrile probe stock solution) 50mL.

[0090] (2)Ag + Preparation of stock solution: Weigh 90.4 mg of silver perchlorate monohydrate, dissolve it in ultrapure water, and prepare 10 mL of a solution with a concentration of 20 mM.

[0091] (3) Other metal ions Li + , Na + , K + , Mg 2+ , Ca 2+ , Ba 2+- , Hg 2+ ,Sr 2+ , Zn 2+ , Al 3+ , Fe 3+ ,Co 2+ , Ni 2+ , Cu 2+ , Zn 2+ , Pb 2+ , Cd 2+ , Mn 2+ Preparation of the stock solution: Take the perchlorate of the corresponding metal ion r...

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Abstract

The invention designs and synthesizes a fluorescent probe capable of identifying Ag+. The Ag+ fluorescent probe is a thiacalixarene derivative, and the structure of the Ag+ fluorescent probe is represented by the following shown compound A (as shown in the description). When the compound having the above structural characteristics serves as the Ag+ fluorescent probe, Ag+ ions can be selectively identified through fluorescence quenching at high selectivity and high sensitivity, the fluorescence energy of a system can be restored after I-ions are further added, namely a Ag<+> fluorescent probe solution can produce an ON-OFF-ON reversible fluorescence phenomenon with alternative adding of Ag<+> / I<->, the reversible process undergoes several switching cycles, and its fluorescence is almost notlost so that the fluorescent probe can be used for achieving continuous reversible cycle detection and is friendly to the environment. In addition, according to remarkable color change of the Ag<+> fluorescent probe, we can easily detect Ag<+> by naked eyes.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, in particular to an Ag + Fluorescent probe and preparation method thereof, a kind of Ag + Green chemical sensor. Background technique [0002] Fluorescent probes have been widely used in the fields of biochemistry, analytical chemistry, and environmental science in recent years due to their high sensitivity, high selectivity, real-time fast response, no damage to samples, and easy manipulation. It is a kind of non-biological "molecular device", which usually consists of a recognition group connected to a fluorescent signal response group through a connecting arm. The recognition group will selectively recognize and detect the object to be analyzed, and then transfer this recognition and detection behavior to the fluorescent signal response group through the connecting arm, and the fluorescent signal response group will send the probe to the probe in a highly sensitive signal response...

Claims

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Application Information

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IPC IPC(8): C07D413/14C09K11/06G01N21/64
CPCC07D413/14C09K11/06C09K2211/1029C09K2211/1048C09K2211/1092G01N21/64G01N21/6428G01N21/643G01N2021/6421G01N2021/6432
Inventor 赵江林金宗文阮琴陈震旻罗擎颖吴玉田
Owner SHENZHEN INST OF ADVANCED TECH
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