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Effective culture and differentiation medium for indica rice anther

A technology for differentiation culture medium and culture medium, applied in the field of agricultural science, can solve the problems of low efficiency of anther culture of indica rice, low differentiation rate of callus green seedlings, limitation of efficient application of indica rice, etc., so as to improve the differentiation rate of green seedlings and improve rice The effect of flower cultivation efficiency and metabolic rate improvement

Inactive Publication Date: 2018-11-06
YIELEAD RICE IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: indica rice anther callus green seedling differentiation rate is low, resulting in low indica rice anther culture efficiency, limiting its high-efficiency application in indica rice breeding

Method used

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  • Effective culture and differentiation medium for indica rice anther
  • Effective culture and differentiation medium for indica rice anther
  • Effective culture and differentiation medium for indica rice anther

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Prepare the culture medium with the following formula:

[0017] KNO 3 1950mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 171mg / L, CaCl 2 2H 2 O 455mg / L, MgSO 4 ·7H 2O 370mg / L, EDTA disodium 37.5mg / L, FeSO 4 ·7H 2 O 27.5mg / L, MnSO 4 4H 2 O 22.5mg / L, ZnSO 4 ·7H 2 O 9mg / L, H 3 BO 3 6.3mg / L, KI 0.83mg / L, CuSO 4 ·5H 2 O 0.025mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CoCl 2 ·6H 2 O 0.025mg / L, Inositol 100mg / L, Glycine 2.0mg / L, Methionine 180mg / L, Niacin 0.75mg / L, Leucine 3.0mg / L, Valine 18mg / L, Vitamin B 1 0.3mg / L, vitamin B 6 0.6mg / L, vitamin E 0.5mg / L, vitamin B 12 3.0mg / L, naphthaleneoxyacetic acid 0.25mg / L, p-aminobenzoic acid 0.5mg / L, sucrose 45g / L, calcium pantothenate 5mg / L, AgNO 3 8mg / L, seaweed polysaccharide 5g / L, NAA 3.5mg / L, ZT 3.0mg / L, IBA 4.0mg / L, 2-IP 2.0mg / L, salicylic acid 0.8mg / L enzyme hydrolyzed casein 1.0g / L L, pH 5.8.

[0018] The anther calli of the Indica rice varieties Xianhui 207, 9311, and Minghui 63 were selected as donors. When the...

Embodiment 2

[0021] Prepare the culture medium of following formula (the preferred addition ratio of phytohormone):

[0022] KNO 3 1950mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 171mg / L, CaCl 2 2H 2 O 455mg / L, MgSO 4 ·7H 2 O 370mg / L, EDTA disodium 37.5mg / L, FeSO 4 ·7H 2 O 27.5mg / L, MnSO 4 4H 2 O 22.5mg / L, ZnSO 4 ·7H 2 O 9mg / L, H 3 BO 3 6.3mg / L, KI 0.83mg / L, CuSO 4 ·5H 2 O 0.025mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CoCl 2 ·6H 2 O 0.025mg / L, Inositol 100mg / L, Glycine 2.0mg / L, Methionine 180mg / L, Niacin 0.75mg / L, Leucine 3.0mg / L, Valine 18mg / L, Vitamin B 1 0.3mg / L, vitamin B 6 0.6mg / L, vitamin E 0.5mg / L, vitamin B 12 3.0mg / L, naphthaleneoxyacetic acid 0.25mg / L, p-aminobenzoic acid 0.5mg / L, sucrose 45g / L, calcium pantothenate 5mg / L, AgNO 3 8mg / L, seaweed polysaccharide 5g / L, salicylic acid 0.8mg / L enzyme hydrolyzed casein 1.0g / L, PH 5.8.

[0023] The concentration ratios of the four plant hormones are shown in Table 1.

[0024] The final concentration ratio of four kind...

Embodiment 3

[0029] Prepare the medium of the following formula (niacin and concentration preferred):

[0030] KNO 3 1950mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 171mg / L, CaCl 2 2H 2 O 455mg / L, MgSO 4 ·7H 2 O 370mg / L, EDTA disodium 37.5mg / L, FeSO 4 ·7H 2 O 27.5mg / L, MnSO 4 4H 2 O 22.5mg / L, ZnSO 4 ·7H 2 O 9mg / L, H 3 BO 3 6.3mg / L, KI 0.83mg / L, CuSO 4 ·5H 2 O 0.025mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CoCl 2 ·6H 2 O 0.025mg / L, Inositol 100mg / L, Glycine 2.0mg / L, Methionine 180mg / L, Leucine 3.0mg / L, Valine 18mg / L, Vitamin B 1 0.3mg / L, vitamin B 6 0.6mg / L, vitamin E 0.5mg / L, vitamin B 12 3.0mg / L, naphthaleneoxyacetic acid 0.25mg / L, p-aminobenzoic acid 0.5mg / L, sucrose 45g / L, calcium pantothenate 5mg / L, AgNO 3 8mg / L, seaweed polysaccharide 5g / L, NAA 3.5mg / L, ZT 3.0mg / L, IBA 4.0mg / L, 2-IP 2.0mg / L, salicylic acid 0.8mg / L enzyme hydrolyzed casein 1.0g / L L, pH 5.8. The concentration gradient of niacin was set to 0, 0.25, 0.5, 1.0, 1.25, 1.5mg / L respectively. Also select thre...

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Abstract

The invention discloses an effective culture and differentiation medium for indica rice anther. The formula of the medium comprises the following components in a certain proportion: KNO3, NH4NO3, KH2PO4, CaCl2.2H2O, MgSO4.7H2O, disodium EDTA, FeSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, Na2MoO4.2H2O, CoCl2.6H2O, inositol, glycine, methionine, niacin, leucine, valine, vitamin E, vitamin B1, vitamin B6, vitamin B12, naphthyloxyacetic acid, salicylic acid, AgNO3, p-aminobenzoic acid, sucrose, calcium pantothenate, algal polysaccharides, NAA, ZT, 2-IP, IBA and casein hydrolysate. Bythe medium, the advantage of differentiation rate of green seedlings of callus of the indica rice anther is improved remarkably, and the culture efficiency of the indica rice anther can be improved remarkably.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a high-efficiency indica rice anther culture and differentiation medium. Background technique [0002] Rice anther culture (flower culture) is also called haploid culture, that is, the process of taking rice anthers or pollen grains at a specific period, in vitro culture, inducing small spores to form callus, and differentiating into haploid plants. The haploid chromosomes of pollen plants are doubled into diploids through artificial or natural methods, and finally achieve the purpose of quickly obtaining pure lines. Anther culture has the advantages of high selection efficiency, stable offspring genetics, rich genetic types, and short breeding period. It is an ideal material for transgenics, molecular markers, and gene map construction. Efficient means of breeding new varieties. Applying anther culture to resistance breeding, since the diploid pollen plants d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
CPCA01H4/001C12N5/04
Inventor 乔保建乔琪任代胜夏祥华付锡江陶元平彭冲
Owner YIELEAD RICE IND
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