Microbiological integrity index-based wetland ecosystem health evaluation method

Abstract

The invention relates to a microbiological integrity index-based wetland ecosystem health evaluation method. The method comprises the following steps: 1) selecting multiple wetlands which can completely reflect the overall water quality and the aquatic ecosystem condition in the to-be-evaluated area, adopting the multiple wetlands as sampling points, and measuring 13 indexes; 2) filtering water samples, shearing a filtered filter membrane, transferring the samples into a crushing tube for DNA extraction, and carrying out PCR (polymerase chain reaction) amplification on obtained DNA samples; distinguishing the samples throughdifferent barcode sequences, distributing the total sequencing reading to all the samples, removing fuzzy bases included in the sequences, removing sequences with the primer mismatching conditionand containing more than six basic groups at the same time, filtering out low-quality sequences, and removing a primer sequence; 3) applying the weighted average regressionmethod to obtainan optimal value of each classification unit, and then determining the sensitive or tolerance classification unit of each key environmental factor. According to the method disclosed bythe invention, based on the biological parameter distribution range, the correlation analysis and the discrimination capability analysis, an M-IBI-based wetland ecosystem health evaluation system isobtained.

Description

technical field [0001] The invention belongs to the technical field of wetland ecosystem protection, and in particular relates to a wetland ecosystem health evaluation method based on microbial integrity index. Background technique [0002] The wetland ecosystem is located between the aquatic and terrestrial ecosystems, known as the transitional zone, which undertakes important functions such as flood regulation and water storage, reduction of pollutant concentration and water conservation, and is known as the most ecological value and diversity. One of the richest areas. However, with the development of social urbanization and the intensification of environmental pollution, on the one hand, the area of ​​wetlands has decreased, and on the other hand, the structure of wetland ecosystems has been destroyed, leading to functional decline, which is manifested as a decrease in the diversity of wetland ecosystems. These changes will affect the ecological health and safety of reg...

AI Technical Summary

Problems solved by technology

At present, there are few studies at home and abroad that use M-IBI for wetland ecosystem health assessment. Therefore, introducing M-IBI into wetland assessment will be a supplement and improvement to the wetland ecosystem health assessment system.

Images