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Complex microbial inoculant for efficiently treating marine petroleum sewage and preparation method of complex microbial inoculant

A compound bacterial agent and offshore oil technology, applied in the field of environmental microorganisms, can solve problems such as difficulty in adapting to the high-salt environment of marine or port oil sewage, and achieve the effect of reducing oil content

Active Publication Date: 2018-10-09
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These petroleum hydrocarbon-degrading bacteria come from fresh water or soil, and it is difficult to adapt to the high-salt environment of marine or port oil sewage, which has considerable limitations for the treatment of ship oil sewage

Method used

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  • Complex microbial inoculant for efficiently treating marine petroleum sewage and preparation method of complex microbial inoculant
  • Complex microbial inoculant for efficiently treating marine petroleum sewage and preparation method of complex microbial inoculant
  • Complex microbial inoculant for efficiently treating marine petroleum sewage and preparation method of complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 prepares petroleum sewage treatment microbial bacterial agent of the present invention

[0038] Strain activation culture: Inoculate the glycerol tube strains of each strain stored at -80°C into a 15mL culture tube filled with 2-5mL HLB liquid medium, and place it in a shaking shaker at 20-30°C and 180-220r / min Cultivate for 48-120 hours, then streak the activated bacterial solution in HLB solid medium and place in a constant temperature incubator at 20-30°C for rejuvenation and culture for 24-96 hours. The 15mL culture tube of the medium was cultured overnight (12-16h) in a shaking shaker at 20-30°C and 180-220r / min, inoculated in S medium (seed liquid medium) with 1% inoculum, and placed in Cultivate in a shaking shaker at 20-30°C, 180-220r / min for 12-18h, then transfer to S medium at 0.5-2.5% inoculum size (20-30°C, 180-220r / min) for 10- After 15 hours, the seed solution of each strain was obtained.

[0039] Preparation of microbial bacterial agent for...

Embodiment 2

[0040] Embodiment 2 Functional Evaluation of Microbial Inoculant for Petroleum Sewage Treatment According to the Present Invention

[0041] Functional evaluation of a single bacterial strain: the 4 strains described in the invention were respectively activated and cultivated according to the method of Example 1, and the bacterial cells were collected by centrifugation at 5000-8000 rpm for 3-6 minutes, and the collected bacterial cells were respectively An equal volume of S medium was resuspended, respectively inoculated in D medium at a ratio of 0.5-2.5%, and the system was cultured in a constant temperature shaker at 20-30°C and 180-220r / min. Starting from 0h, sampling every 24h, continuous sampling for 3 times, after extraction with organic solvent, GC-MS is used to detect the remaining amount of oil.

[0042] The method for evaluating the function of microbial bacterial agents for petroleum sewage treatment according to the present invention is: the 4 strains of bacteria de...

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Abstract

The invention belongs to the field of environmental microorganisms and particularly relates to a complex microbial inoculant for efficiently treating marine petroleum sewage and a preparation method of the complex microbial inoculant. The complex microbial inoculant comprises four strains, namely alcanivorax jadensis, alcanivorax venustensis, acinetobacter venetianus and alteromonas macleodii. Different strains in the inoculant exert different functions in treatment of petroleum sewage, cooperate with each other and promote mutually, so that the oil content, comprising a large amount of various linear hydrocarbon, branched hydrocarbon and aromatic hydrocarbon existing in the petroleum, of the petroleum sewage is obviously reduced, and industrialization and commercialization of the microbial inoculant for efficiently treating the marine petroleum sewage are facilitated.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a microbial agent for treating marine petroleum sewage and a preparation method thereof. Background technique [0002] Most of the oil pollution occurs in the marine environment, which is extremely harmful to the ocean and its surrounding ecological environment. It not only causes the lack of oxygen in large areas of the sea, but also kills fish, shrimp, shellfish and seabirds. The oil slick pollutes the beach and causes beach pollution. Desolation, destroying mariculture and salt pan production, destroying coastal tourist areas, and reducing the service function and value of marine ecosystems; in addition, many petroleum hydrocarbons, especially polycyclic aromatic hydrocarbons, can also enter fish, shrimp, and shellfish through the food chain, affecting other fish, shrimp, and shellfish. economic value, and eventually enter the human body through food enr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC12N1/20C02F3/34C02F2101/32
Inventor 何敬愉崔金明蒙海林刘陈立傅雄飞何彩云
Owner GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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