Alligator sinensis microsatellite polymorphic site and identification method and specific primer sequence thereof

Abstract

The invention provides an alligator sinensis microsatellite polymorphic site and an identification method and specific primer sequences thereof. On this basis, thirteen pairs of specific primer sequences are screened, and the sequences are shown in SEQ ID NO.1-SEQ ID NO.26. The method for identifying the alligator sinensis microsatellite polymorphic site comprises the steps that an alligator sinensis genome is adopted to screen microsatellite sites and high-quality primers, sequencing read data of the alligator sinensis genome is compared to an alligator sinensis reference genome to identify INDEL, according to an INDEL identification result and a microsatellite screening result, the microsatellite sites with polymorphism are screened out. The provided thirteen pairs of specific primers are all polymorphic sites and have stronger resolution capability. The set of microsatellite primers can be economically, quickly and accurately used for the paternity test of the alligator sinensis, thereby being conductive to performing phylon reconstruction and information perfecting on alligator sinensis population, so that a scientific management scheme is formulated to guide the later protection work of the alligator sinensis.

Description

Technical field [0001] The invention belongs to the field of identification of polymorphic sites of Chinese alligator microsatellites, and specifically relates to polymorphic sites of Chinese alligator microsatellites, identification methods and specific primer sequences. Background technique [0002] Chinese alligator, commonly known as Tulong and Zhubolong, was listed as critically endangered by the Red List of the World Conservation Union (IUCN) in 1996. It is a rare reptile unique to China. Chinese alligators were once widely distributed throughout the country. As time went by, the northern boundary of the Chinese alligators gradually moved south. By the middle of the 19th century, alligators were mainly distributed in the middle and lower reaches of the Yangtze River. The next 100 years will be a period of rapid decline in the Chinese alligator population. A survey at the beginning of the 20th century showed that there were less than 130 wild Chinese alligators, mainly conc...

AI Technical Summary

Problems solved by technology

However, the previous method of screening microsatellites using species reference genomes has two defects: 1. The specificity of primer design is poor, which is prone to non-specific amplification; Spending more time and cost for further screening

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