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Method for detecting rot disease infection of poplar

A technology of rot disease and poplar, which is applied in the field of detection of poplar rot infection, can solve problems such as the death of shelter belts and the threat of oasis ecological agriculture, and achieve the effect of preventing large-scale occurrence

Inactive Publication Date: 2018-09-28
TARIM UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

In recent years, poplar rot disease has spread rapidly, resulting in the death of a large area of ​​shelterbelts, posing a great threat to the fragile oasis ecological agriculture

Method used

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  • Method for detecting rot disease infection of poplar
  • Method for detecting rot disease infection of poplar
  • Method for detecting rot disease infection of poplar

Examples

Experimental program
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Effect test

Embodiment 1

[0069] In April 2017, 100 poplars with the same growth conditions were selected and no rot was observed. When the external conditions did not change significantly, 50 fresh leaves in the middle of the poplar were selected for enzyme activity determination. 50 The bark tissue of the strains was taken to measure the enzyme activity.

[0070] The specific operation steps of enzyme activity assay are as follows:

[0071] A: Determination of peroxidase (POD) activity

[0072] The activity of peroxidase is determined by using a peroxidase kit, the steps are:

[0073] (1) Preparation of crude enzyme solution:

[0074] According to the mass volume ratio of 1g: 10mLg / mL, add 0.1g leaf or bark tissue to 1mL extract, homogenize in ice bath, centrifuge at 4°C and 8000g centrifugal force for 10 minutes, and take the supernatant , to obtain a crude enzyme solution; put the crude enzyme solution on ice for testing;

[0075] (2) After preheating the crude enzyme solution and reagent at 25...

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Abstract

The invention discloses a method for detecting rot disease infection of a poplar, and belongs to the field of rot diseases. The method for detecting rot disease infection of the poplar comprises the following steps that the activity of enzymes in the poplar is detected, and whether the poplar is infected with a rot disease or not is judged according to the change of the enzyme activity; and the enzymes are one or more of a peroxidase, a catalase, a superoxide dismutase, beta-1,3-dextranase and a phenylalnine ammonialyase. According to the method for detecting rot disease infection of the poplar, when the external conditions are not changed greatly, one or more of the peroxidase, the catalase, the superoxide dismutase, the beta-1,3-dextranase and the phenylalnine ammonialyase are subjectedto enzyme activity detection, and whether the poplar is infected with the rot disease or not is judged according to the change of the enzyme activity; and the invasion period and the incubation periodof the rot disease can be effectively detected, and the large area of the poplar rot disease can be prevented in advance.

Description

technical field [0001] The invention belongs to the field of rot disease, and in particular relates to a method for detecting poplar infected with rot disease. Background technique [0002] Poplar is one of the main shelterbelt tree species in Xinjiang, which plays an extremely important role in the agricultural production of oasis. In recent years, poplar rot has spread rapidly, resulting in the death of a large area of ​​shelterbelts and posing a great threat to the fragile oasis ecological agriculture. In the early stage of rot infection, poplar trees have no visible symptoms, but when obvious symptoms appear in the later stage, it is too late to prevent them. Therefore, the detection of the pre-invasion and incubation period of rot disease has become a key technology to prevent the occurrence of rot disease. [0003] In view of this, the present invention proposes a method for detecting poplar rot infection, which can effectively detect the invasion period and incubati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12Q1/28C12Q1/30C12Q1/34C12Q1/527
Inventor 张王斌朱宗财刘振亚严海璘岳娟仪子博王飞亚
Owner TARIM UNIV
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