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Long-wavelength ultra-sensitive carbon monoxide colorimetric fluorescent probe

A technology of compound and branched alkoxy group, which is applied in the field of long-wavelength ultrasensitive carbon monoxide colorimetric fluorescent probes, can solve the problems of insufficient response speed, complex synthesis, and short emission wavelength, so as to facilitate popularization and application and simple synthesis , good stability

Inactive Publication Date: 2018-09-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with the currently reported colorimetric and fluorescent probes, including insufficient selectivity, insufficient sensitivity, insufficient response speed, short emission wavelength, and complex synthesis.

Method used

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  • Long-wavelength ultra-sensitive carbon monoxide colorimetric fluorescent probe
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  • Long-wavelength ultra-sensitive carbon monoxide colorimetric fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]

[0034] (Scheme 1) Dissolve 387 (1 mmol) cyanine fluorophore compound in 15 mL of dichloromethane, then add 120 mg (1 mmol) allyl chloroformate and 101 mg (1 mmol) triethylamine, then The reaction was stirred at 25° C. for 3 h, and after the reaction was completed, the dichloromethane was evaporated to dryness to obtain the crude product. Finally, a mixed system of dichloromethane and petroleum ether (3:1, v / v) was used for column chromatography to obtain 292 mg of a yellow pure product with a yield of 62%.

[0035] (Scheme 2) Dissolve 387 (1 mmol) cyanine fluorophore compound in 15 mL of dichloromethane, then add 144 mg (1.2 mmol) allyl chloroformate and 101 mg (1 mmol) triethylamine, then The reaction was stirred at 25° C. for 3 h, and after the reaction was completed, the dichloromethane was evaporated to dryness to obtain the crude product. Finally, a mixed system of dichloromethane and petroleum ether (3:1, v / v) was used for column chromatography to obtain 306 m...

Embodiment 2

[0040] figure 1 It is the change graph of the fluorescence spectrum after adding carbon monoxide (0-40 μM) to the probe (5 μM). Take 50μL from the probe mother solution and put it into 10mL test system (containing 10μM Pb 2+ , PBS 7.4, 5mM), configure multiple parallel samples. Then carbon monoxide of different concentrations (different volumes were pipetted from 1 millimolar carbon monoxide mother liquor) was added into the test system, shaken evenly and left to stand for 20 minutes. Fluorescence intensity changes were measured with a fluorescence spectrophotometer. It can be clearly seen from the figure that with the increase of the concentration of carbon monoxide added, the fluorescence intensity at 605 nm of the solution gradually increases. Moreover, it can be seen from the illustration that the fluorescence intensity at 605nm has a good linear relationship with the added carbon monoxide concentration (0-1000nM), which proves that the quantitative analysis of carbon m...

Embodiment 3

[0042] image 3 It is the change graph of the fluorescence spectrum with time after the probe (5 μM) is added with carbon monoxide (30 μM). Take 50 μL from the probe mother solution and place it in 10 mL of the test system (containing 10 μM Pd 2+ , PBS 7.4, 5mM). Then 30 μM carbon monoxide (250 μL was pipetted from 1 mM carbon monoxide mother solution) was added into the test system, and the fluorescence intensity change was measured with a fluorescence spectrophotometer immediately after shaking evenly. It can be clearly seen from the figure that when carbon monoxide is added, the fluorescence intensity reaches the maximum value and remains unchanged after 30 minutes of detection, which shows that the probe reacts rapidly with carbon monoxide and can provide a rapid analysis method for the determination of carbon monoxide.

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Abstract

The invention relates to a long-wavelength ultra-sensitive carbon monoxide colorimetric fluorescent probe. Particularly, the probe is a cyanine compound, and can be used as a carbon monoxide ratio fluorescent probe for detecting carbon monoxide. The probe has at least one of the following technical effects: the carbon monoxide is recognized at high selectivity; the probe can rapidly respond the carbon monoxide; the carbon monoxide can be analyzed at high sensitivity; colorimetric analysis on the carbon monoxide can be realized; the property is stable, and the probe can be stored and used for along time; and the anti-jamming capability is high.

Description

technical field [0001] The invention relates to a cyanine compound used as a colorimetric fluorescent probe for carbon monoxide, which can quickly perform ultrasensitive and selective recognition of carbon monoxide, or it can measure the concentration of carbon monoxide in a sample. Background technique [0002] Carbon monoxide (CO) is a product of incomplete combustion of carbon-containing substances such as coal and petroleum. It is a colorless, odorless, non-irritating toxic gas. It is almost insoluble in water and does not easily react with other substances in the air. Therefore, it can stay in the atmosphere for 2 to 3 years. If the local pollution is serious, it will be harmful to the health of the crowd. The background concentration of carbon monoxide in the troposphere is about 0.1-2ppm, which is harmless to the human body. Due to the continuous development of transportation, industrial and mining enterprises in various countries in the world, the consumption of fu...

Claims

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Application Information

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IPC IPC(8): C07D307/68C09B23/14C09K11/06G01N21/64G01N21/78
CPCC07D307/68C09B23/145C09K11/06C09K2211/1007C09K2211/1088G01N21/6428G01N21/78
Inventor 王作凯柳彩云耿卓凡吕小钰王瑞康朱宝存
Owner UNIV OF JINAN
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