Gene markers for screening obsessive-compulsive disorder, schizophrenia, and depression and their applications
A technology for schizophrenia and obsessive-compulsive disorder, applied in the field of molecular biology, can solve the problems affecting the symptomatic treatment of mental diseases, expression differences, and poor diagnostic consistency.
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Embodiment 1
[0022] Example 1 Screening of characteristic gene markers for obsessive-compulsive disorder
[0023] Screening for trait genes for obsessive-compulsive disorder includes the following steps:
[0024] 1) Using the Affymetrix Gene Expression Using Affymetrix U133Plus 2.0 human whole gene expression profile chip to analyze the peripheral blood gene expression profile of 20 cases of obsessive-compulsive disorder patients, 20 cases of schizophrenia and 20 cases of depression patients, the above 60 samples were used as training set (Training set);
[0025] 2) Using the Affymetrix MAS5 method to normalize the gene expression profile chip detection results of each sample in the training set;
[0026] 3) Eliminate the probe set (Probeset) signals with excessively high and low expression signals in the total RNA, and select the probe set signals with moderate expression in all 60 samples of the training set for subsequent analysis;
[0027] 4) T-test method was used to select the diff...
Embodiment 2
[0033] Example 2 Using the screened characteristic gene markers of obsessive-compulsive disorder to screen and diagnose obsessive-compulsive disorder, schizophrenia, and depression
[0034] 1. Method steps:
[0035] 1) Collection of peripheral blood samples of samples to be tested: use BD PAXgeneRNA blood collection tubes from QIAGEN to collect peripheral blood samples from patients;
[0036]2) Extraction and purification of total RNA in the peripheral blood sample of the sample to be tested: use QIAGEN's PAXgeneBloodRNA Kit to extract and purify the total RNA in the peripheral blood, and use the Agilent BioAnalyzer 2100 microelectrophoresis analyzer to identify the integrity and yield of the extracted total RNA fragments;
[0037] 3) Reverse transcription reaction: use Life Techonolgy's High-Capacity cDNA ReverseTranscription kit reverse transcription kit, use total RNA as a template, and use Olig(dT) as a reverse transcription primer to perform a reverse transcription reacti...
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