Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Haplotype combination relevant to Mediterranean buffalo good milk secretion property and application

A buffalo and lactation technology, applied in the direction of recombinant DNA technology, microbe determination/inspection, DNA/RNA fragments, etc.

Active Publication Date: 2018-09-07
HUAZHONG AGRI UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(Mukherjee R, Yun J W. Long chainacyl CoA synthetase 1 and gelsolin are oppositely regulated in adipogenesis and lipogenesis. [J]. Biochem Biophys ResCommun, 2012, 420 (3): 588-593.) There is no ACSL1 gene in molecular markers or buffalo Research reports on lactation traits, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Haplotype combination relevant to Mediterranean buffalo good milk secretion property and application
  • Haplotype combination relevant to Mediterranean buffalo good milk secretion property and application
  • Haplotype combination relevant to Mediterranean buffalo good milk secretion property and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1: Identification of ACSL1_SNP1 to ACSL1_SNP12 markers associated with lactation traits in Mediterranean buffaloes and primer design for genotype detection at ACSL1_SNP1 to ACSL1_SNP12 loci

[0118] The Mediterranean river buffalo was selected for the test, and according to the buffalo ACSL1 genome sequence (accession number Gene ID: 102414095), primer pairs with SEQ ID NO: 2-21 respectively amplifying the target fragment of the gene were designed, and the above-mentioned Mediterranean buffalo genomic DNA was used as a template, as follows Procedure for PCR amplification:

[0119] The PCR reaction system is shown in Table 1.

[0120] Table 1 PCR reaction system

[0121] Taq Mix

10μl

Sense Primer (10mM)

0.5μl

Anti-Sense Primer (10mM)

0.5μl

Genomic DNA (50-100ng / μl)

1μl

ddH2O

8μl

Total Volume

20μl

[0122] The PCR reaction conditions are shown in Table 2.

[0123] Table 2PCR reaction condi...

Embodiment 2

[0128] Example 2: Application of Markers ACSL1_SNP1 to ACSL1_SNP12 in Association Analysis of Buffalo Lactation Traits

[0129]In order to determine whether the detected Mediterranean buffalo markers ACSL1_SNP1 to ACSL1_SNP12 are related to the differences in the milking phenotype of buffaloes, purebred Mediterranean buffaloes (331 heads) were selected as test materials in this example, and all data were compiled by the Italian Breeding Association (AIA) and Italian Agricultural Courtesy of the Council on Research (CAR). The polymorphism was detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and the correlation between different genotypes and lactation traits of buffalo was analyzed. The mixed linear model (Mixed) in R language statistical software was used for correlation analysis between genotype and phenotype values, and the analysis model was:

[0130] Y ijkl =u+G i +P j +S k +F l +PS jk +PF jl +SF kl +e

[01...

Embodiment 3

[0146] Example 3: Identification of haplotype combinations from ACSL1_SNP1 to ACSL1_SNP 12 with excellent lactation traits

[0147] 1. Construction of haplotype and haplotype combination

[0148] The haplotype analysis of ACSL1_SNP1 to ACSL1_SNP12 was performed using Haploview software. Input the obtained genotype data of ACSL1_SNP1 to ACSL1_SNP12 loci of all individuals into the PHASE program, calculate the genotype of each individual, and calculate the degree of pairwise linkage disequilibrium between the loci. Haplotype block analysis such as Figure 14 shown.

[0149] Depend on Figure 14 It can be seen that according to the linkage disequilibrium analysis of ACSL1_SNP1 to ACSL1_SNP12, this application found 2 haplotype blocks: block1 is a total of 2 SNPs from ACSL1_SNP1 to ACSL1_SNP2 with strong linkage; block2 is a total of 9 SNPs from ACSL1_SNP4 to ACSL1_SNP12 The degree of dot linkage is strong.

[0150] The following is based on block2 as haplotype analysis.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of livestock molecular marker screening, and particularly relates to a haplotype combination relevant to the Mediterranean buffalo good milk secretion property and application. The molecular marker is obtained through the clone from the Mediterranean buffalo; 12 SNP markers relevant to the milk secretion property are obtained through screening; the haplotype formed by one marker or the combined markers is used for performing relevance analysis on the Mediterranean buffalo milk secretion property. The sequences of the 12 SNP markers of the ACSL1 geneare shown by SEQ ID NO: 1 to SEQ ID NO: 12. The invention also relates to development of detection primer of ACSL1_SNP1 and ACSL1_SNP12 site genotypes and application of good haplotype combined sequences. The haplotype combined sequences are obviously relevant to the Mediterranean buffalo peak milk quantity, 270d milk yield and 270d milk protein quantity properties. The invention provides a novelgenetic marker resource for the marker auxiliary selection of the Mediterranean buffalo.

Description

technical field [0001] The invention belongs to the technical field of buffalo molecular marker screening and auxiliary selection. It specifically involves the SNP markers and their haplotype combinations and applications related to the excellent lactation traits of Mediterranean buffaloes. The present invention screens out 12 SNP markers located on the ACSL1 gene (referred to as ACSL1_SNP1 to ACSL1_SNP12) associated with the lactating traits of the Mediterranean buffalo. Application of association analysis for lactation traits in Mediterranean buffalo. Background technique [0002] Buffalo is the second largest milk-producing livestock in the world and one of the important livestock species in southern my country. Buffalo milk is rich in nutrients such as high milk fat, milk protein, minerals and vitamins, and its nutritional value far exceeds that of milk produced by Holstein cows. Although buffalo milk enjoys the reputation of "the king of milk", its low milk yield gre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 滑国华孙晖邓廷贤杨利国魏鹏飞邵宝顺张燊杜超
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com