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A rapid propagation method of virus-free Rehmannia glutinosa test-tube seedlings by micro-cutting

A test-tube seedling and micro-cutting technology, applied in the directions of vegetative reproduction, botanical equipment and methods, horticulture, etc., can solve the problems of low operation efficiency, easy death, and high cost, and achieve low cost, robust virus-free seedlings, and simple operation. Effect

Active Publication Date: 2020-08-18
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low operation efficiency, high cost and easy death in existing rehmannia test-tube seedling propagation, the present invention provides a rapid micro-cutting propagation method of detoxified rehmannia test-tube seedlings

Method used

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  • A rapid propagation method of virus-free Rehmannia glutinosa test-tube seedlings by micro-cutting
  • A rapid propagation method of virus-free Rehmannia glutinosa test-tube seedlings by micro-cutting
  • A rapid propagation method of virus-free Rehmannia glutinosa test-tube seedlings by micro-cutting

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Experimental program
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Embodiment 1

[0026] A method for rapid propagation of detoxified rehmannia test-tube seedlings by micro-cuttings, the following steps are adopted: a. Cut the detoxified rehmannia rehmannia test-tube seedlings into 2 cm bud stem sections on a super-clean workbench, and then move them into Cultivate in the culture medium of 5.6, the culture temperature is 25°C, the light intensity is 2500Lx, and the light time is 8 hours / day; b. When the detoxified Rehmannia glutinosa test-tube plantlets are cultivated for 30 days or grow to more than 10cm, they should be placed in an open environment. Use scissors at an angle of 45 degrees to cut a budding stem section with one leaf and one bud, and immediately soak it in tap water at 10°C for 2 minutes; c. Insert the soaked budding stem section into a seedling tray equipped with vermiculite , two stems with buds in each hole, the cutting positions are distributed in a diagonal shape, and before the stems with buds grow new buds, the humidity in the seedling...

Embodiment 2

[0029] A method for rapid propagation of detoxified rehmannia test-tube seedlings by micro-cuttings, the following steps are adopted: a. Cut the detoxified rehmannia rehmannia test-tube seedlings into 2.5 cm bud stem segments on an ultra-clean workbench, and then move them into sugar-containing 30g / L, PH Cultured in a culture medium with a value of 6.4, the culture temperature was 28°C, the light intensity was 3000Lx, and the light time was 8 hours / day; b. When the detoxified Rehmannia glutinosa test-tube plantlets were cultivated for 35 days or grew to more than 10cm, they were placed in an open environment Next, use scissors at an angle of 45 degrees to cut a budding stem section with one leaf and one bud, and immediately soak it in tap water at 25°C for a soaking time of 8 minutes; In each hole, there are two stem sections with buds, and the cutting positions are distributed in a diagonal shape. Before the stem sections with buds grow new buds, the humidity in the seedling t...

Embodiment 3

[0032] A method for rapid propagation of detoxified rehmannia test-tube seedlings by micro-cuttings. The following steps are adopted: a. Cut the detoxified rehmannia rehmannia test-tube seedlings into 2.1 cm bud stem sections on a super-clean workbench, and then move them into sugar-containing 30g / L, PH The value is 5.9 culture medium, culture temperature is 27 ℃, light intensity is 2699Lx, and light time is 8 hours / day; Next, use scissors at an angle of 45 degrees to cut into a budding stem section with one leaf and one bud, and immediately soak it in tap water at 19°C for 5 minutes; In each hole, there are two stems with buds, and the cutting positions are distributed in a diagonal shape. Before the stems with buds grow new buds, the humidity in the seedling tray is 92%, the temperature is 22°C, and the light intensity under the film is 1846Lx; d. After the cuttings grow new shoots and roots, they are transplanted into the field to complete rapid propagation.

[0033] The h...

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Abstract

The invention particularly relates to a micro-cuttage rapid propagation method of a virus-free rehmannia glutinosa test-tube plantlet, and solves the problems of low operation efficiency, high cost and easy death in existing rehmannia glutinosa test-tube plantlet propagation. The method comprises the following steps: a, cutting the virus-free rehmannia glutinosa test-tube plantlet into stems withbuds, and carrying out culture, wherein the culture temperature is 25-28 DEG C, the illumination intensity is 2500-3000 Lx, and the illumination time is 8 hours / day; b, in an opening environment, cutting the stems with buds into stem segments with buds, each of which has one leaf and one bud, and immediately soaking the stem segments with buds into tap water; c, carrying out cuttage in a seedlingbreeding tray, wherein each hole is provided with two stem segments with buds, the cuttage positions are in diagonal distribution, and before the stem segments with buds grow new buds, the moisture inthe seedling breeding tray is 90-95%, the temperature is 15-30 DEG C, and the under film illumination intensity is 1500-2000 Lx; and d, after the cuttage seedlings grow new buds and roots, transplanting the cuttage seedlings to a field, so that the rapid propagation is completed. With the adoption of the method provided by the invention, the propagation amount is greatly improved, and the cost ofrapid propagation of the virus-free rehmannia glutinosa test-tube plantlet is reduced.

Description

technical field [0001] The invention relates to a method for propagating test-tube seedlings in the agricultural planting technology, in particular to a micro-cutt rapid propagation method for virus-free rehmannia rehmannia test-tube seedlings. Background technique [0002] Rehmannia glutinosa is used in a large amount of Chinese medicinal materials, and the annual planting area in my country is about 600,000 mu. According to the survey, the planting area of ​​Rehmannia glutinosa in southern Shanxi currently accounts for more than 50% of the planting area of ​​​​Rehmannia glutinosa in my country. However, Rehmannia glutinosa has been asexually propagated by roots for a long time in production, and is harmed by pathogenic bacteria such as Rehmannia degeneration virus (DDV), Rehmannia X virus and CMV, which degrades the species of Rehmannia glutinosa and seriously affects the yield and quality. For a long time, there have been research institutes or universities successively p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/10
CPCA01G2/10
Inventor 左静静闫贵云霍利光王慧杰刘少翔闫建俊左敏
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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