A separation device for extracting platelets from whole blood

Abstract

The invention relates to the field of medical experiment instruments and in particular discloses a separation device for extracting blood platelet from whole blood. The separation device comprises a layering test tube, a rotating disc, a centrifugal mechanism and an oscillation mechanism, wherein the rotating disc is used for clamping and fixing the layering test tube; the centrifugal mechanism isused for driving the rotating disc to rotate; the oscillation mechanism is used for driving the rotating disc to carry out intermittent oscillation in the rotation process. The separation device is high in separation efficiency and simple to operate, and in addition, blood platelet can be thoroughly separated from substances such as other blood cells and plasma proteins in whole blood.

Description

technical field [0001] The invention relates to the field of medical experiment equipment, in particular to a separation device for extracting platelets from whole blood. Background technique [0002] Platelets are one of the formed components in mammalian blood, and are biologically active small pieces of cytoplasm that are lysed and shed from the cytoplasm of mature megakaryocytes in the bone marrow. Small in size, non-nuclear, irregular in shape, and 2-3 microns in diameter, platelets have long been regarded as non-functional cell fragments in the blood. [0003] With the advancement of clinical medicine and medical experimentation, more and more attention has been paid to the blood platelet component in whole blood, and the global clinical demand for platelets is increasing faster than that of red blood cells. This is mainly from the progress of clinical treatment methods. For example, radiotherapy and chemotherapy, bone marrow transplantation, massive blood transfusio...

AI Technical Summary

Problems solved by technology

On the one hand, because the platelet layer is located in the middle of this method, the extraction is difficult, the loss rate is high, and the purity is low; on the other hand, the limited platelets in the whole blood will be more or less The blood cells stay in the plasma or permeate into the lower layer of blood cells. Under the premise of shortage of whole blood resources, it is necessary to purify the platelets in the plasma and blood cells many times, which makes the separation efficiency of blood cells low, cumbersome to operate, and the loss is large, which cannot meet the requirements. The increasing demand for the amount of platelets used in experiments; the practice of blindly increasing the centrifugation speed to obtain high-purity platelets during the centrifugation process is likely to cause the activation of platelets, because activated platelets can affect future experimental information and data, and even change the results, often with half the effort

There is still a certain amount of plasma protein in platelets separated by the above two methods. When performing fluorescence analysis or other analysis, the existence of plasma protein will have a certain interference effect on the experiment.

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