A kind of Aspergillus parasitica spore compound insecticide and preparation method thereof
A technology of Aspergillus parasiticus and Aspergillus spores, which is applied in the field of Aspergillus parasiticus spore compound insecticide and its preparation, which can solve the problems of not being broad-spectrum, limiting the scope of use, and not lasting, so as to avoid adverse effects and improve the insecticidal effect and insecticidal efficiency, the effect of controlling reproduction
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Embodiment 1
[0023] The present embodiment provides a compound insecticide for Aspergillus parasitica spores, which is a wettable powder, and by mass percentage, it includes 5.5% calcium lignosulfonate, 2.5% sodium fluorescein, 1.5% oxalic acid, 2.5% % polyethylene glycol, 3% spore germination accelerator, 5% rotenone powder and 80% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator includes 0.15% peptone, 0.05% yeast powder, 0.1 % glucose, 0.1% ammonium sulfate, 0.1% dipotassium hydrogen phosphate, 95% diatomaceous earth.
[0024] The preparation method of described Aspergillus parasitica spore compound insecticide comprises the following steps:
[0025] 1) Preparation of Aspergillus parasitica spore powder: transfer the Aspergillus parasitica species to PDA liquid medium, and place it on a shaker and cultivate it at 28°C for 24 hours to make liquid mycelium seeds. The inoculum amount was inoculated into a solid medium composed of cornmeal, bran...
Embodiment 2
[0033] The present embodiment provides a kind of Aspergillus parasitica spore compound insecticide, the difference between the present embodiment and embodiment 1 is: by mass percentage, it comprises 5% calcium lignosulfonate, 2% sodium fluorescein, 1% oxalic acid, 2% polyethylene glycol, 2% spore germination accelerator, 5% rotenone powder and 83% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator includes 0.1% peptone, 0.03% yeast powder , 0.05% glucose, 0.05% ammonium sulfate, 0.15% dipotassium hydrogen phosphate, 96.4% diatomaceous earth.
Embodiment 3
[0035]The present embodiment provides a kind of Aspergillus parasitica spore compound insecticide, the difference between the present embodiment and embodiment 1 is: by mass percentage, it comprises 6% calcium lignosulfonate, 3% sodium fluorescein, 2% oxalic acid, 3% polyethylene glycol, 4% spore germination accelerator, 5% rotenone powder and 77% spore powder (Aspergillus parasiticus RWSF001-02); wherein, the spore germination accelerator 0.2% peptone, 0.07% yeast powder, 0.15% glucose, 0.15% ammonium sulfate, 0.15% dipotassium hydrogen phosphate, 97% diatomaceous earth.
[0036] performance verification
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